TISTD反应器中的菌群结构及生态变化  

Microbial Community Structure and Ecological Variation in TISTD Reactor

作　　者：叶姜瑜[1,2] 丁维[1,2] 何强[1,2] 孙兴福[1,2] 

机构地区：[1]重庆大学城市建设与环境工程学院,重庆400045 [2]重庆大学三峡库区生态环境教育部重点实验室,重庆400045

出　　处：《土木建筑与环境工程》2011年第1期147-152,共6页Journal of Civil,Architectural & Environment Engineering

基　　金：科技部国际合作项目(2007DFA90660);国家水体污染控制与治理科技重大专项(2008ZX07315-003)

摘　　要：以两相一体式污泥浓缩消化反应器(Two-phase Integrated Sludge Thickening andDigestion reactor,TISTD)为对象,研究了反应器稳定运行期间菌群结构及反应器负荷改变时其内部生态结构的变化。在以30%的投配率稳定运行时,从产酸相和产甲烷相分别取样、培养和分离出20株优势菌属。选取其中6株进行了16S rDNA序列分析,分属于芽孢杆菌属、产甲烷螺菌属和产甲烷球菌属。在投配率分别为10%、20%、30%以及启动期和系统崩溃期5个时期采取10组样品,对其污泥进行DNA提取、纯化和16S rDNA PCR扩增及温度梯度凝胶电泳(Temperature gradientgel electrophoresis,TGGE)电泳检测等。结果表明:中温下反应器中微生物菌群呈多样性分布,生态系统复杂稳定,维持了反应器的正常功能;反应器不同负荷时期污泥DNA提取及扩增效果良好;TGGE图谱分析表明,投配率20%、30%时的群落结构相似度最高,污泥中生物含量丰富,支撑了TISTD在污泥浓缩下良好的消化效果。Based on the sewage treatment device of Two-phase Integrated Sludge Thickening and Digestion reactor（TISTD）,microbial community structure in stable operation and its ecological variation when load changes are investigated.During a stable operation with 30% sludge dosage rate,20 dominant bacterial strains are individually isolated from acid-phase and methane-phase of the reactor.Sequence analysis of 16S rDNA reveals that six of the achieved strains belong to Bacillus,Methanospirillum and Methanococcus respectively.Ten sludge samples are collected separately in 5 periods when the reactor operates with 10%,20% and 30% sludge dosage rate and in the state of started-up and disordered,and followed by DNA extraction,purification,16S rDNA PCR amplification and temperature gradient gel electrophoresis（TGGE）.The good quality of DNA extraction and amplification demonstrates that there is an abundant biomass in the sludge under mid-temperature condition.The results show that TISTD reactor has a high biodiversity in microbial communities and is complex and stable in ecosystem structure,which effectively supports the good digestion in sludge thickening.

关 键 词：TISTD 菌群结构 温度梯度凝胶电泳 多样性 16SrDNA 生态系统 

分 类 号：X172[环境科学与工程—环境科学]