高效液相色谱串联质谱检测地表水中五种生物胺  

Determination of Five Biogenic Amines in Surface Water by HPLC-MS/MS

作　　者：程小艳[1] 杨秋红[2] 雷鄂蓉 张渝[1] 王英英[1] 

机构地区：[1]四川省环境监测中心站,成都610041 [2]四川大学建筑与环境学院,成都610065

出　　处：《四川环境》2011年第1期35-39,共5页Sichuan Environment

摘　　要：采用固相萃取及高效液相色谱串联质谱技术,建立了地表水中5种生物胺(腐胺、尸胺、2-苯乙胺、酪胺、色胺)的测定方法。水样中2-苯乙胺、酪胺和色胺可直接进样测定,腐胺和尸胺经HLB固相萃取柱富集,乙腈洗脱,氮吹浓缩至1.0mL后进样液相色谱串联质谱分析,以选择离子监测(SRM)模式定量分析。在本实验条件下,加标回收率在89.4%～122%之间,相对标准偏差2.15%～11.9%(n=7),检出限在0.05～5μg/L。A novel method for determination of five biogenic amines in surface water with solid-phase extraction and HPLC-MS/MS was developed.The biogenic amines 2-phenylethylamine,tyramine and tryptamine in water were directly injected into LC-MS/MS.As to putrescine and cadaverine in water sample,before injection,they were concentrated by HLB extraction column,then eluted with acetonitrile eluants.The solvent was concentrated by nitrogen blow to 1.0mL.Quantities were analyzed by SRM mode.At the conditions of this experiment,the spiked recoveries and relative standard deviations（RSD,n=7） were 89.4%～122% and 2.15%～11.9% respectively,The detection limit was 0.05～5μg/L.

关 键 词：生物胺 高效液相色谱串联质谱 固相萃取 地表水 

分 类 号：X832[环境科学与工程—环境工程]