杀菌剂在莲瓣兰原生地萌发胚培养中的应用  

Research on Fungicide in the Culture of Cymbidium tortisepalum's Germination Embryo in situ

作　　者：李海峰[1] 赵志莲 胡侃[1] 李明[1] 

机构地区：[1]大理学院药学院,云南大理671000 [2]云南省大理农业学校,云南大理671003

出　　处：《北方园艺》2011年第3期148-150,共3页Northern Horticulture

基　　金：云南省大理州科技局2007年资助项目;大理学院药物研究所资助项目(2009yy02)

摘　　要：以莲瓣兰杂交种子原生地播种萌发突破种皮后的胚为外植体,研究了培养基中添加不同种类、不同浓度的杀菌剂对胚培养过程中真菌污染的控制作用。结果表明:培养基中添加培福朗、苯菌灵和代森锰锌能有效控制莲瓣兰杂交种子原生地萌发突破种皮后的胚培养过程中的真菌污染,胚污染率可以控制在10.0%以内,培养基中添加培福朗时胚成活率显著高于添加苯菌灵和代森锰锌,胚成活率高达92.4%;培养基中培福朗浓度在0.5～1.0mg/L范围内时胚污染率能够控制在9.0%以内,胚成活率超过92.3%,不仅能有效控制莲瓣兰杂交种子原生地萌发突破种皮后的胚培养过程中真菌污染,还能促进胚生长发育转绿成根状茎。Using the seeding germination embryo of Cymbidium tortisepalum＇s hybrid seeds in situ as explants,to observe the effects of different kinds,different concentration of fungicide on the control of fungus pollution during the process of culturing the germination embryo of Cymbidium tortisepalum＇ s hybrid seeds.The results showed that uazation, benomyl,mancozeb in medium could control fungus pollution in germination embryo of Cymbidium tortisepalum＇ s hybird seeds in situ,by using uazatine,the fungus pollution percent of embryo was controlled within 10.0%, significantly greater than benomyl and mancozeb group.The survival ratio of embryo was 92.4%,the concentration ranges of uazatine were 0.5～1.0 mg/L.The fungus pollution percent of germination embryo of Cymbidium tortisepalum＇s hybird seeds in situ was controlled within 9.0%,the survival ratio of embryo remarkably higher than 92.3%,not only effectively controlled fungus pollution in the course of culturing germination embryo hybrid seeds of Cymbidium tortisepalum in situ,but also promoted embryo growth and transformed into rhizome.

关 键 词：莲瓣兰 胚培养 杀菌剂 真菌污染 

分 类 号：S682.29[农业科学—观赏园艺] S603.6[农业科学—园艺学]