GC法测定灵丹油清咽喷雾剂中β-芹子烯的含量  

Determination of β-selinene in Lingdanyou Qingyan Spray by GC

作　　者：胡玉霞[1] 李家平 余启荣[1] 高柏丽[1] 高茗 和芳[1] 

机构地区：[1]北京创立科创医药技术开发有限公司,北京100029 [2]云南盘龙云海药业有限公司

出　　处：《中国药事》2011年第1期33-35,共3页Chinese Pharmaceutical Affairs

基　　金：国家十一五科技支撑计划项目(编号2006BAI06A18-01)

摘　　要：目的建立灵丹油清咽喷雾剂中β-芹子烯的含量测定方法。方法采用GC法,色谱柱为HP-5MS毛细管柱;检测器为FID;程序升温：初始温度80℃,保持2min,以每分钟2℃的速率升温至150℃,再以每分钟10℃的速率升温至200℃,保持8min。结果β-芹子烯在0.036875~0.4425μg范围内线性关系良好（r=0.9999,n=5）,平均回收率为98.44%,RSD为2.03%。结论本方法简便,重复性好,专属性强,可用于灵丹油清咽喷雾剂的质量控制。Objective To establish a method for determination of β-selinene in Lingdanyou Qingyan Spray by GC.Methods The GC was equipped with FID detector,and a HP-5MS capillary column was used.The temperature program of column oven was as follows：80℃,held for 2min,increased at a rate of 2℃·min-1 to 150℃,then at 10℃·min-1 to 200℃,held for 8min.Results The results showed a good linearity（r=0.9999） between 0.036875～0.4425μg.The average recovery was 98.44% and RSD 2.03%.Conclusion The method is simple,accurate and specific.It can be used for the quality control of Lingdanyou Qingyan Spray.

关 键 词：灵丹油清咽喷雾剂 β-芹子烯 GC 

分 类 号：R284[医药卫生—中药学] R927.2[医药卫生—中医学]