武陵山野生红菇液体培养条件及产胞外多糖研究  

Submerged Culture Conditions and Exopolysaccharides Production of Wild Russula Lepida Fr.in Wuling Mountain

作　　者：陈今朝[1] 王剑锋 秦家顺[1] 谭永忠[1] 

机构地区：[1]长江师范学院生命科学与技术学院,重庆408100 [2]东华理工大学生物系,南昌344000

出　　处：《河南师范大学学报（自然科学版）》2011年第1期158-161,共4页Journal of Henan Normal University(Natural Science Edition)

基　　金：重庆市科委科技攻关项目(CSTC;2006AC1045);重庆市教委科研项目(KJ101304)

摘　　要：对武陵山野生红菇进行液体摇瓶培养,以菌丝体干重为筛选指标,得到最适合野生红菇生长的培养条件为可溶性淀粉3.0%,蛋白胨0.60%,硫酸镁0.10%,磷酸二氢钾0.20%,初始pH 6.5,培养温度28℃,转速180 r.min-1,装液量75 mL,250 mL-1三角瓶,培养8 d.此时,菌丝体干重达0.605 3 g.100 mL-1,红菇的胞外粗多糖产量达到0.546 1 g.l00 mL-1.The dry weight of the mycelia of the wild Russula lepida Fr.in Wuling Mountain are screened by submerged cultivating.The optimum culture conditions of the wild Russula lepida Fr.growth are as follows： 3.0% soluble starch,0.60% peptone,0.10%MgSO4·7H2O,0.20%KH2PO4,the original pH value is 6.5,the optimal temperature is 28 ℃,the optimal rotational speed is 180 r·min-1,the optimal medium capacity is 75 mL.250 mL-1 triagical bottle,and the optimal cultivating time are 8 days.Under these culture conditions,the dry weight of the mycelia and the production of exopolysaccharides from the wild Russula lepida Fr.are up to 0.605 3 g·100 mL-1 and 0.546 1 g·l00 mL-1 respectively.

关 键 词：野生红菇 液体培养 菌丝体 孢外多糖 

分 类 号：S646.19[农业科学—蔬菜学]