漆酶/ABTS介质系统对蒽醌染料的脱色效果  

Decolorization Effect of Anthraquinone Dye with Laccase/ABTS Mediator System

作　　者：孙秋芳[1,2] 赵亮亮[1,2] 李燕荣[3] 谌斌[1,2] 

机构地区：[1]广西环境工程与保护评价重点实验室,广西桂林541004 [2]广西师范大学环境与资源学院,广西桂林541004 [3]中国农业科学院农业资源与农业区划研究所,北京100081

出　　处：《贵州农业科学》2011年第1期190-193,共4页Guizhou Agricultural Sciences

基　　金：广西环境工程与保护评价重点实验室研究基金(桂科能0702Z022);广西环境工程与保护评价重点实验室平台建设项目[教科研(2008)27];广西研究生教育创新计划项目(2009106020830M66)

摘　　要：为提高蒽醌类染料废水的脱色率,以红芝(Ganoderma lucidum)发酵所得漆酶粗酶液与ABTS组成漆酶/ABTS介质系统对蒽醌染料活性艳蓝KN-R进行了脱色试验,分别考察不同pH、温度、ABTS加入量、酶加入量、金属离子等条件对活性艳蓝KN-R脱色的影响。结果表明:最优脱色条件为pH 4.0,温度30℃,ABTS浓度10μmol/L,酶液用量为30 U/mL;此条件下对400 mg/L活性艳蓝KN-R进行脱色反应60 min,脱色率达92%以上;漆酶/ABTS脱色活性艳蓝的动力学参数Km值为3 556 mg/L,最大反应速率Vmax为91.7mg/(L.min)。The effects of pH,temperature,metal ion and dosage of ABTS and enzyme on the decolorization effect of anthraquinone dye KN-R were studied in the test of decoloration of anthraquinone dye KN-R with mediator system,which was composed of laccase from Ganoderma lucidum and ABTS,to enhance decoloration rate of anthraquinone dye wastewater.Results showed that The optimal conditions included pH 4.0,temperature 30℃,ABTS concentration 10 μmol/L and laccase 30U/mL.the decoloring rate for 400 mg/L KN-R was over 92% in 60 minutes under the optimal conditions.The Km of anthraquinone dye KN-R with laccase/ABTS was 3 556 mg/L and Vmax was 91.7 mg/（L.min）.

关 键 词：红芝 漆酶/ABTS介质系统 蒽醌染料 脱色 

分 类 号：X788[环境科学与工程—环境工程]