CDH12对涎腺腺样囊性癌细胞侵袭能力的影响  

Effect of CDH12 on the Invasive Ability of Salivary Adenoid Cystic Carcinoma Cells

作　　者：王锦锋[1] 佘林[1] 于林灿[1] 郑斐斐[1] 卢友光[1] 

机构地区：[1]福建医科大学附属口腔医院预防科,福州350002

出　　处：《中国医科大学学报》2011年第1期33-37,共5页Journal of China Medical University

基　　金：福建省科技厅科技计划重点项目(2008Y0041)

摘　　要：目的探讨CDH12基因对涎腺腺样囊性癌细胞(SACC)侵袭能力的影响。方法应用巢式RT-PCR法从涎腺腺样囊性癌高转移细胞株SACC-M的总RNA中扩增出CDH12cDNA;应用AdEasyTMXL腺病毒载体系统构建含人CDH12基因的重组腺病毒表达载体;转染SACC-M细胞后,采用Western blot检测CDH12蛋白分子的表达,采用侵袭小室法检测CDH12对SACC-M细胞侵袭能力的影响。结果 CDH12基因重组腺病毒及空腺病毒经鉴定正确;Western blot检测发现,CDH12基因重组腺病毒转染细胞(Ad-CDH12/SACC-M)组CDH12的表达水平明显高于空腺病毒载体转染(Ad0/SACC-M)对照组;与Ad0/SACC-M组相比,Ad-CDH12/SACC-M组细胞的侵袭能力明显高于Ad0/SACC-M组(P<0.01)。结论 CDH12能够促进涎腺腺样囊性癌细胞的体外侵袭能力,提示该基因在涎腺腺样囊性癌的侵袭转移中可能具有重要意义。Objective To clarify the effect of gene CDH12 on invasiveness of human salivary adenoid cystic carcinoma cells.Methods CDH12 cDNA was amplified by nested RT-PCR using the total RNA extracted from the highly metastatic human salivary adenoid cystic carcinoma cell line SACC-M.The CDH12 gene recombinant adenoviral expressing vector was constructed using AdEasyTM XL Adenoviral Vector System.SACC-M cells were infected with recombinant adenovirus and the expressed target proteins were examined by Western blot.Tumor invasion assay was performed to study the effect of CDH12 on invasiveness of SACC-M cells.Results The CDH12 gene recombinant and control adenoviral expressing vector were successfully constructed.The expression of CDH12 was significantly increased in Ad-CDH12/SACC-M cells compared with that in Ad0/SACC-M cells.Compared with Ad0/SACC-M cells,the invasive ability of Ad-CDH12/SACC-M cells had been enhanced significantly.Conclusion CDH12 could increase the invasion of salivary adenoid cystic carcinoma cells in vitro.CDH12 might have an important role in the invasion and metastasis of salivary adenoid cystic carcinoma.

关 键 词：CDH12 腺病毒 腺样囊性癌 侵袭 

分 类 号：R781.7[医药卫生—口腔医学]