当归补血汤载药血清对骨髓基质细胞增殖及细胞因子表达的影响  

Effect of Serum Containing Drug of Danggui Buxue Decoction on the Proliferation of Mice Bone Marrow Stromal Cells and Expression of Cytokines

作　　者：王晓玲[1] 汪涛[1] 汪雅妮[1] 赵舒武[2] 王学谦[2] 

机构地区：[1]天津中医药大学中医学院组织学与胚胎学教研室,天津300193 [2]天津医院,天津300193

出　　处：《辽宁中医杂志》2011年第2期363-365,共3页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然基金面上项目(30772881);天津市高等学校科技发展基金计划项目(20060301)

摘　　要：目的:观察当归补血汤载药血清对小鼠骨髓基质细胞增殖及细胞因子分泌的影响。方法:分离骨髓基质细胞,接种于96孔板培养,在相差显微镜下观察细胞形态和生长状况;将细胞分4组,用含有不同剂量的当归补血汤载药血清作用,采用MTT法检测各组细胞的增殖活性及ELISA法检测造血生长因子GM-CSF和IL-3的表达情况。结果:在不同浓度的载药血清作用下,骨髓基质细胞增殖和分泌GM-CSF和IL-3显著(P<0.05),呈剂量依赖性,但浓度过高可减弱细胞的增殖分泌效应。结论:当归补血汤载药血清能够促进骨髓基质细胞的增殖以及GM-CSF和IL-3的分泌。Objective：To investigate the effect of serum containing drug of Danggui Buxue Decoction on the proliferation of mice bone marrow stromal cell（MBSCs） and expression of cytokines.Methods：MBSCs of the new born mouse were procured.MBSCs were cultured in the 96 holes board by using the method of serum-free incubation technique and the growth characteristic of cells was observed.MBSCs were divided into 4 groups and then MBSCS were cultured at different concentrations in the serum containing drug of Danggui Buxue Decoction.MTT method was used to observe the effects of various culture fluid on proliferation of MBSCs and ELISA method was used to observe the expression level of hematopoietic growth factors GM-CSF and IL-3.Results：After the stimulation of various culture fluid,the results suggested MBSCs showed a proliferative property manifesting a higher cell number.The expression level of GM-CSF and IL-3 of each serum containing drug of Danggui Buxue Decoction group was significantly higher than the normal control.All had significant difference.But with heightener of the concentration of Danggui Buxue Decoction the proliferation of MBSCs and the expression level of GM-CSF and IL-3 were weakened.Conclusion：The serum containing drug of Danggui Buxue Decoction promote the proliferation of MBSCs significantly and the expression level of GM-CSF and IL-3.

关 键 词：当归补血汤 骨髓基质细胞 造血微环境 增殖 细胞因子 

分 类 号：R-33[医药卫生]