两种环境下甘蓝型油菜含油量的差值QTL分析  

Analysis of Difference QTLs for Oil Content between Two Environments in Brassica napus L.

作　　者：李超[1,2,3] 李波[1,3] 曲存民[1,3] 阎星颖[1,3] 付福友[1,3] 刘列钊 谌利[1,3] 李加纳[1,3] 

机构地区：[1]西南大学农学与生物科技学院,重庆400716 [2]贵州省油料研究所,贵州贵阳550006 [3]重庆市油菜工程技术研究中心,重庆400716

出　　处：《作物学报》2011年第2期249-254,共6页Acta Agronomica Sinica

基　　金：国家科技支撑计划项目(2009BADA8B01);国家自然科学基金项目(31071450);重庆市攻关项目(CSTC;2010AA1014);贵州省农业科学院创新基金项目[黔农科合(创新基金)2010007号]资助

摘　　要：利用本实验室构建的遗传连锁图谱和复合区间作图法检测重组自交系GH06×P174（SWU-1）和GH06×中油821（SWU-2）群体在2个环境中含油量差值的QTL。以SWU-1群体在2个环境中检测到2个含油量差值QTL,分别位于2个不同的连锁群,单个QTL可解释表型变异的10.31%-12.45%;以SWU-2群体在2个环境中检测到3个含油量差值QTL,分别位于2个不同的连锁群,单个QTL可解释表型变异的6.60%-10.58%。分析结果表明,含油量受环境影响较大,差值的变异幅度达到0-18.66个百分点,变异系数达到58.24%,说明在油菜的油脂合成中,存在对环境敏感和钝感的基因。含油量差值QTL与2个环境中分别检测到的含油量QTL没有明显的连锁关系,初步分析说明对环境敏感或钝感的基因与油脂合成基因不是同一个系统。Based on the genetic linkage maps of Brassica napus L.established by our laboratory,QTLs for oil content difference between two environments were detected using composite interval mapping model.The mapping populations,SWU-1 and SWU-2,were planted in Wanzhou,Chongqing with high latitude and Beibei,Chongqing with low latitude in 2005,which contained 188 and 219 recombinant inbred lines,respectively.In the SWU-1 population,two difference QTLs were located in the linkage groups 2 and 7,which explained 10.31%-12.45% of the phenotypic variation each.In the SWU-2 population,three difference QTLs were located in linkage groups 8 and 14,and a single QTL explained 6.60%-10.58% of the phenotypic variation.The oil content difference of genotypes ranged from 0 to 18.66% with coefficient of variation of 58.24%.This indicates the presence of environment-insensitive QTLs associated with oil content in rapeseed genotypes.In both environments,no visible linkage relationship was observed between QTLs for oil content difference and the QTLs for oil content.Therefore,it is inferred that sensitive and insensitive genes to environment might have a different genetic expression system to oil synthesis genes in Brassica napus.

关 键 词：甘蓝型油菜 含油量 环境 差值QTL 

分 类 号：S565.4[农业科学—作物学]