三种基因转移方法在胶质瘤的基因治疗中的应用  

The Application of Three Gene Transfer Systems in the Growth Inhibition of Glioma Cell by HSV TK Gene

作　　者：房欣[1] 杨虎山[1] 王金环[1] 冯宇新 史国利[1] 

机构地区：[1]天津南开大学分子生物学研究所分子遗传研究室,300071

出　　处：《中国肿瘤临床与康复》1999年第4期40-42,共3页Chinese Journal of Clinical Oncology and Rehabilitation

摘　　要：目的　为基因治疗寻找有效的基因转移方法。方法　以腺病毒为载体，以聚阳离子化合物介导和直接应用裸质粒这三种基因转移方法，观察了转染以后神经胶质母细胞瘤细胞对ＧＣＶ药物的敏感性。结果　应用ＧＣＶ ７２小时后，腺病毒载体组细胞生存率仅为２３％ ，而聚阳离子化合物介导的基因转移组和直接应用裸质粒组的细胞生存率分别为５７％ 和９６％ ，远远高于腺病毒载体组。结论　腺病毒介导的基因转移方法是胶质瘤基因治疗的有效手段。? Objective To find effective gene transfer methods for gene therapy of glioma.Methods The plasmid pACCMV TK which expresses herpes simple virus thymidine kinase(HSV TK)gene was constructed,and recombinant adenovirus AdCMV TK was gained.Glioma cell TJ905 was treated with AdCMV TK,polybrene/pACCMV TK and plasmid pACCMV TK respectively and their GCV sensitivity was observed.Results Proliferation of TJ905 cells infected with AdCMV TK was inhibited evidently.The cell survival rate was 23% in the third day after GCV was used,which was remarkably lower than those of the cells treated with polybrene/pACCMV TK(57%) and plasmid pACCMV TK(96%).Conclusion Recombinant AdCMV TK is a feasible gene therapeutic tool for glioma. 〔

关 键 词：重组腺病毒 聚阳离子化合物 胶质瘤 基因治疗 

分 类 号：R730.51[医药卫生—肿瘤] R730.264[医药卫生—临床医学]