食管鳞癌DNA和RNA测定及其预后关系的研究  

DNA and RNA content and their prognostic significance in esophageal squamous cell carcinoma

作　　者：卢兆桐[1] 尹格平[1] 孙晓明[1] 袁黎军 李浩[1] 

机构地区：[1]济南军区总医院胸外科,250031

出　　处：《中国肿瘤临床与康复》1999年第4期43-43,42,共2页Chinese Journal of Clinical Oncology and Rehabilitation

摘　　要：目的　探讨食管鳞癌ＤＮＡ异倍体和ＲＮＡ含量变化与预后的关系。方法　应用流式细胞技术对６２例随访患者的石蜡标本进行研究。结果　术后生存５年以上患者的ＤＮＡ异倍体７８．１％（２５／３２），ＲＮＡ含量为２０．５３±４．２１。术后半年内死亡者ＤＮＡ为９３．３％ （２８／３０），ＲＮＡ含量为２４．８４±６．３５，两者均有显著性差异。ＤＮＡ异倍体和ＲＮＡ含量与病期、恶性程度和淋巴结转移有关。结论　ＤＮＡ和ＲＮＡ测定是判断食管鳞癌病期、恶性程度、有无淋巴结转移和预后的重要指标。表２　食管癌不同病期与ＤＮＡ和ＲＮＡ含量的关系生存时间（年） 病期 例数 ＤＮＡ２Ｃ Ａｎ ％ＲＮＡ含量（ｘ±ｓ）＞ ５ Ⅰ ３ ２ １ ５０ １０．６１±０．２４Ⅱ １９ ４ １５ ７８．９ ２１．５６±３．４１Ⅲ ８ １ ７ ８７．５ ２３．９８±２．１１Ⅳ ２ ０ ２ １００ ２６．１２±１．５６＜ ０．５ Ⅰ ０ ０ ０Ⅱ １１ １ １０ ９０．９ ２３．４５±４．２７Ⅲ １３ １ １２ ９２．３ ２４．７６±２．８７Ⅳ ６ ０ ６ １００ ２７．３１±２．５６表３　食管鳞病分化程度与ＤＮＡ和ＲＮＡ含量的关系生存时间（年） 分化 例数 ＤＮＡ２Ｃ Ａｎ ％ＲＮＡ含量（ｘ±ｓ）＞ ５ Ⅰ ５? Objective To study DNA and RNA content and their prognostic significance in esophageal squamous cell carcinoma.Methods Using flow cytometry (FCM) technique,to study DNA and RNA content in 62 patients with esophageal squamous cell carcinoma.Results The incidence of DNA heteroploidy was 78.1% in patients survived more than 5 years postoperation,93.3% in patients died 6 months postoperation,showing a remarkable difference (P<0.05);the RNA content was 20.53±4.21 in patients survived more than 5 years postoperation,24.84±6.35 in patients died 6 months postoperation These two data also showed a remarkable difference(P<0.05).The incidence of DNA heteroploidy and RNA content were related to the stage,malignant degree and the incidence of lymph node metastasis.The incidence of DNA heteroploidy and RNA content in patients survived more than 5 years were lower than these in patients died 6 months postoperation.Conclusion DAN and RNA content is an useful quantitative indicator in determining the stage,malignant degree. 〔

关 键 词：组织 食管肿瘤 DNA RNA 流式细胞术 

分 类 号：R735.107[医药卫生—肿瘤]