胆囊癌肝脏转移动物模型的实验研究  

作　　者：童洪飞[1] 张伟[1] 林海舵[1] 倪仲琳[1] 游涛[1] 林胜璋[1] 

机构地区：[1]温州医学院附属第二医院,325000

出　　处：《浙江临床医学》2011年第2期121-123,共3页Zhejiang Clinical Medical Journal

基　　金：温州市科技局基金资助项目（Y20070090）浙江省卫生厅医药卫生研究基金资助项目（2008QN023）浙江省科技基础条件平台研究项目（2009P82G2090001）

摘　　要：目的建立胆囊癌肝脏转移动物模型，并对其进行初步评估。方法用脾内接种法建立人胆囊癌细胞系（GBC—SD）的裸鼠肝脏转移模型。接种后第45天行肝脏CT检查，并处死裸鼠后取下肝脏行病理检查。结果CT检查肝转移率为40％（4／10），探查发现肝转移率为50％（5／10），CT检查的阳性率为80％（4／5），组织病理学证实肝脏瘤结节均为转移瘤。结论脾内接种法可以成功建立胆囊癌肝脏转移动物模型，CT可以作为初步评估该模型的影像学工具。Objective To establish and evaluate liver metastasis model of gallbladder cancer in nude mice for further clinical trial. Methods The gallbladder carcinoma cells GBC - SD were injected into the spleen of nude mouse. After 45 days, the mice were scanned by computerized tomography and sacrificed for pathological examination. Results The liver metastasis rate was 50% （5/10） pathologically. Four mice with metastasis were detected by CT scan. The positive rate of CT scan was 80% （4/5）. Conclusion The results suggest that the liver metastasis model of gallbladder carcinoma in nude mice can be established through spleen injection and evaluated by CT initially.

关 键 词：裸鼠 人胆囊癌细胞 肝转移 CT 

分 类 号：R735.2[医药卫生—肿瘤]