食线虫真菌的筛选及菌株DF09002的鉴定与产酶特性分析  

Screening and identification of nematophagous fungi and enzymatic activity assay

作　　者：曾庆飞[1,2] 黄惠琴[1] 朱军[1] 方哲[1] 吴晓鹏[1] 孙前光[1] 鲍时翔[1] 

机构地区：[1]中国热带农业科学院热带生物技术研究所,海口571101 [2]海南大学农学院,海口570228

出　　处：《植物保护》2011年第1期41-44,49,共5页Plant Protection

基　　金：中央级公益性科研院所基金(ITBBZX2008-1-2);海南省研究生创新课题基金(Hxwby2009-06);博士科研启动基金(ITBBqd0801)

摘　　要：从海南五指山原始森林、定安雷鸣发病胡椒园采集土壤样品及死亡线虫尸体,分离得到126株菌落形态有差异的真菌。以离体培养的南方根结线虫为靶标线虫对126株真菌进行筛选,获得7株对根结线虫校正死亡率在70%以上的菌株。菌株DF09002抗线虫活性最强,校正死亡率达100%;经5次继代培养,仍具有稳定产生杀线虫活性物质的能力。通过形态学和rDNA ITS序列分析,将该菌株归类为曲霉属黑色组曲霉黑曲霉集合体。酶系分析试验表明,菌株能产生在线虫侵染过程中起重要作用的几丁质酶和胞外蛋白酶,酶活力分别达到0.674、1.63 U/mL。A total of 126 fungal strains were isolated from soil samples collected from Wuzhishan Mountain and pepper orchards in Ding＇an of Hainan Province in China. By using Meloidogyne incognita cultured in vitro as target nematodes for selecting fungi against Meloidogyne, 7 strains were selected, among which the strain DF09002 had the highest nematicidal activity, with its lethal rate as high as 100%. The strain was identified as Aspergillus niger aggregate through morphological observation and rDNA-ITS sequence analyses. Enzymatic assays indicated that the strain DF09002 could produce chitinase and extracellular protease, with their activities as 0. 67 and 41.63 U/ mL, respectively.

关 键 词：根结线虫 食线虫真菌 筛选 菌株鉴定 酶系分析 

分 类 号：S476[农业科学—农业昆虫与害虫防治]