HPLC法测定梅花入骨丹中的没食子酸和槲皮素含量  

Determination of Gallic Acid and Quercetin inBauhinia Championi (Benth.) Benth by HPLC

作　　者：叶蕻芝[1,2] 陈鹏程[3] 杨建萍 陈红青[4] 徐两军[4] 郑春松 刘献祥[1] 

机构地区：[1]福建中医药大学中西医结合研究院,福建福州350108 [2]福建省中西医结合老年性疾病重点实验室,福建福州350108 [3]福建中医药大学附属第二人民医院,福建福州350003 [4]福州大学测试中心,福建福州350002

出　　处：《福建中医药大学学报》2011年第1期35-39,共5页Journal of Fujian College of Traditional Chinese Medicine

基　　金：福建省自然科学基金资助课题(2010Y0032;2009J01172);福建省属高校科研专项资助课题(JK2009016);福建省中医药科研重点课题资助课题(WZZG0901);陈可冀中西医结合发展基金资助课题(2009006)

摘　　要：目的测定梅花入骨丹中没食子酸和槲皮素的含量。方法用HPLC法,以SinoChromC18(4.6 mm×250 mm,5μm)为色谱柱,流动相为乙腈-0.2%磷酸溶液,流速为1 mL/min,检测波长为255 nm,柱温为30℃,梯度洗脱。结果没食子酸在0.162～3.24μg,槲皮素在0.002052～0.041μg内呈良好的线性关系,没食子酸平均回收率为103.9%,RSD为1.20%;槲皮素平均回收率为100.5%,RSD为0.97%。结论 HPLC法测定没食子酸和槲皮素,方法简便可行,重复性和分离效果好。Objective To determine the Championi （Benth.） Benth. Methods The separation contents of gallic acid and quercetin in Bauhinia was performed on SinoChrom C18（4.6mm×250mm,5μm）） with gradient elution of acetonitrile-0.2% phosphoric acid solution as mobile phase, flow rate was 1.0 mL·min^-1, the detective wavelength was 255 nm, and the column temperature was 30℃. Results The linear ranges of gallic acid and quercetin were 0.162-3.24 μg, 0.002052-0.041μg respectively, the average recoveries were 103.9% （RSD= 1.20%）, 100.5% （RSD=0.97%） respectively. Conclusion The method is simple and reproducible, and it has good separation effect.

关 键 词：梅花入骨丹 高效液相色谱 没食子酸 槲皮素 

分 类 号：R284.2[医药卫生—中药学]