柿叶中总黄酮提取工艺模式的建立及抗氧化活性研究  

Extraction of total flavonoids from persimmon leaves and test on its anti-oxidative activity

作　　者：王小芳[1] 董晓宁[1] 刘玉姣[1] 赵强[2] 

机构地区：[1]天水师范学院生命科学与化学学院,甘肃天水741001 [2]甘肃农业大学动物医学院

出　　处：《中兽医医药杂志》2011年第1期32-35,共4页Journal of Traditional Chinese Veterinary Medicine

基　　金：甘肃省天水师范学院科研基金资助项目(TSA0605)

摘　　要：采用黄酮类化合物的吸收波长510 nm为指标,以索氏回流法进行正交实验,将柿叶提取物进行紫外光谱检测,测定总黄酮的含量,对比优选出最佳提取工艺。测定提取的黄酮化合物对超氧负离子自由基O2-.的清除作用。结果表明,乙醇浓度、乙醇用量、回流时间、pH值对柿叶总黄酮的提取均有影响。其最佳提取工艺参数为:乙醇浓度60%、乙醇体积200 mL、回流时间1 h、碱提pH值9;柿叶中黄酮类化合物对清除自由基有一定的作用,且黄酮类化合物的添加量在实验范围内与其抗氧化性呈正相关。Total flavonoids were extracted with Soxhlet reflux method at the maximum absorbance wavelength of 510 nm from persimmon leaves,then were measured after detected by UV spectra.The extracting process was optimized by orthogonal test.The scavenging effect of total flavonoids extracts on superoxide anion radical O2-· was also studied.The optimum process parameters were as follows： 200 mL ethanol of 60%,reflux time of 1 h and pH value adjusted to 9 with alkali.Persimmon flavonoids could scavenge free radicals to some extent,and there was a dose-effect relationship between the additions of flavonoids with its antioxidant activity.

关 键 词：柿叶 总黄酮 正交实验 抗氧化活性 

分 类 号：S853.74[农业科学—临床兽医学]