二氯甲烷高效降解菌株的诱变选育  

Breeding of highly effective dichloromethane degradation strains

作　　者：胡志航[1] 徐士博[1] 祝飞[1] 徐铭[1] 吴石金[1] 

机构地区：[1]浙江工业大学生物与环境工程学院,浙江杭州310032

出　　处：《浙江工业大学学报》2011年第1期7-12,共6页Journal of Zhejiang University of Technology

基　　金：国家自然科学基金资助项目(20977087);浙江省自然科学基金资助项目(Y5090054)

摘　　要：从工厂活性污泥中分离筛选到1株二氯甲烷降解菌WH22,鉴定为梭形芽孢杆菌(Lysiniba-cillus Fusiformis),利用60Coγ辐射和紫外线(UV)进行复合诱变.诱变的最佳条件:菌悬液经0.5 kGy60Coγ射线处理后,紫外照射处理(18 W,距离30 cm)100 s或120 s.菌液经以二氯甲烷为唯一碳源的无机盐培养基筛选,最终筛选到3株正突变株.在二氯甲烷浓度为800 mg/L的水溶液中,3株正突变株二氯甲烷的降解率分别为66.20%,52.90%,52.10%,比原始菌株的降解率分别提高了191.63%,133.04%,129.52%.正交试验表明:温度对突变株的降解效果影响最大,最佳温度为30℃.3株正突变株经8次传代培养,降解效率分别下降12.40%,11.05%,0.68%,是性状较为稳定可深入研究开发的优良菌株.The parent dichloromethane（DCM）-degrading bacterium strain WH22 was isolated from activated sludge of a factory and identified as Lysinibacillus Fusiformis.The strain was induced by 60Coγ and UV for increasing the degration rate.The best dosage of 60Coγ process is 0.5 kGy,and the best dosage of UV process is 100 s or 120 s（18 W,30 cm）.Three of the highly effective DCM degrading mutants were breeded.They were measured in the MSM culture,adding DCM as the sole carbon and energy.With the condition of 800 mg/L DCM,their degration rate were 66.2%,52.9%,52.1%,which were 191.63%,133.04%,129.52% higher than the original strain,respectively.Among the orthogonal test,temperature is the most important factor;the best temperature is 30 ℃.Desecend of 12.40%,11.05%,0.68% in the degration rate of the mutants were observed after 8 times of subculture,respectively.Therefore,the obtained mutants are stable strains that are worth further study.

关 键 词：DCM 60Coγ射线 紫外光射线 生物降解 菌株选育 

分 类 号：S432.4[农业科学—植物病理学]