胰岛素样生长因子结合蛋白-5在肝硬化、原发性肝癌患者肝组织和血清中的水平及临床意义  

Levels and clinical significance of insulin-like growth factor binding protein-5 in cirrhosis and primary liver can-cer

作　　者：毛胜[1] 强晖[1] 肖明兵[1] 周国雄[1] 

机构地区：[1]江苏省南通大学附属医院消化内科,226001

出　　处：《中国临床研究》2011年第2期96-99,共4页Chinese Journal of Clinical Research

摘　　要：目的观察肝硬化、原发性肝癌患者肝组织和血清中胰岛素样生长因子结合蛋白-5(IGFBP-5)水平,探讨其与肝硬化、原发性肝癌的关系及其临床意义。方法取肝硬化组织、肝癌组织及癌旁正常组织各8例,采用实时RT-PCR技术检测IGFBP-5mRNA的表达水平;取肝硬化(21例)、原发性肝癌(14例)及正常对照者(14例)血清,采用ELISA法检测IGFBP-5水平,分析IGFBP-5在不同分组中水平的差异及其临床意义。结果肝硬化组肝组织中IG-FBP-5mRNA的表达及血清中IGFBP-5水平均较正常对照组降低(P均<0.05),与血清中升高的透明质酸(HA)、层粘连蛋白(LN)和肝功能无相关性。原发性肝癌组肝组织中IGFBP-5的表达及血清中IGFBP-5水平均高于正常对照组和肝硬化组(P均<0.05),与血清中升高的甲胎蛋白(AFP)和肝功能亦无相关性。结论在肝硬化发生后,肝组织表达和合成IGFBP-5减少,与其他细胞因子的调节有关,其可能与HA、LN互补作为诊断早期肝纤维化的一个无创指标;原发性肝癌患者肝组织中IGFBP-5表达增多,血清水平增高,其有可能作为一个潜在的诊断原发性肝癌的检测指标。IGFBP-5与肝硬化、原发性肝癌的关系值得进一步研究。Objective To investigate the expressions of insulin-like growth factor binding protein-5（IGFBP-5）mRNA in the liver and serum leves of IGFBP-5 in patients with liver cirrhosis and primary liver cancer for discussing its clinical significance and its possible association with liver cirrhosis and primary liver cancer.Methods IGFBP-5 mRNA expressions in liver cancer tissues,liver cirrhosis and the adjacent normal tissues（n=8 each） were examined by real-time RT-PCR.Serum levels of IGFBP-5 in patients with cirrhosis（n=21）and primary liver cancer（n=14）and healthy subjects（n=14）were detected by ELISA.The difference of the expressions and serum levels of IGFBP-5 in the mentioned above groups and its clinical significance were analyzed.Results The expressions of IGFBP-5 mRNA in liver tissues and serums levels of IGFBP-5 in patients with cirrhosis were significantly lower than those in matched healthy subjects （all P〈0.05）,and the IGFBP-5 levels in serums were not associated with the high levels of AST,ALT,HA and LN in the same cirrhosis group.Meanwhile,the expressions of IGFBP-5 mRNA in liver tissues and serums levels of IGFBP-5 in patients with primary liver cancer were significantly higher than those in the other two groups （all P〈0.05）,and the IGFBP-5 levels in serums were not associated with the high levels of AST,ALT and AFP in the same cancer group.Conclusions The lower IGFBP-5 expression in the liver cirrhosis patients might be closely associated with the regulation of other cytokines in the liver.While the levels of IGFBP-5 may be a noninvasive examine indicator in the diagnosis of the early liver fibrosis associated with HA and LN.The levels of IGFBP-5 may be a potential examine indicator in the diagnosis of primary liver cancer due to it’s high levels in the liver tissues and serums.The further studying about IGFBP-5 may benefit the diagnosis and therapy of liver cirrhosis and primary liver cancer.

关 键 词：胰岛素样生长因子结合蛋白-5 肝硬化 原发性肝癌 逆转录多聚酶链反应 

分 类 号：R575.2[医药卫生—消化系统] R735.7[医药卫生—内科学]