人乳头状瘤病毒感染与乳腺癌的关系及与P16蛋白表达的相关性研究  

HPV infection:correlation with breast cancer and P16 expression

作　　者：刘静[1] 尚凡晶[1] 刘建生[1] 罗飞[2] 

机构地区：[1]山西医科大学第一医院普外科,太原030001 [2]山西省肿瘤医院乳腺外科

出　　处：《中国药物与临床》2011年第2期147-150,共4页Chinese Remedies & Clinics

摘　　要：目的检测乳腺良恶性肿瘤中人乳头状瘤病毒(HPV)16/18DNA和P16蛋白的阳性率,分析HPV16/18感染与抑癌基因P16蛋白表达的相关性以及HPV16/18感染与乳腺癌临床病理特征的相关性。方法对2008年1月至12月山西医科大学第一医院普外科手术切除的35例乳腺癌组织、35例癌旁组织、30例乳腺纤维腺瘤组织以及30例乳腺纤维瘤旁正常组织进行HPV16/18DNA分子原位杂交和P16蛋白免疫组织化学检测。结果乳腺癌组织中HPV16/l8的阳性率为71%,与纤维瘤组织和瘤旁正常组织相比,差异均具有统计学意义;HPV16/18在乳腺癌组织中的表达与腋淋巴结转移呈正相关(r=0.152,P=0.021),与患者月经状况、肿瘤大小无关;HPV16/18DNA和P16蛋白在乳腺癌组织中的表达呈负相关(r=-0.662,P=0.000)。结论 HPV在乳腺癌组织中高表达,对乳腺癌的发生具有病因学意义。HPV感染与乳腺癌发生腋窝淋巴结转移呈正相关,可以作为评价乳腺癌发展和预后的指标。HPV感染与抑癌基因P16的失活在乳腺癌中呈负相关,二者可能在乳腺癌的发病中相互作用,共同影响乳腺癌的发生发展。Objective To determine the positive rate of HPV16/18DNA and P16 protein in benign and malignant breast tumors and to investigate the correlation of HPV16/18 infection with P16 expression and clinical pathological features of breast cancer.Methods Between January 2008 and December 2008,HPV16/18 DNA in situ hybridization and immunohistochemical study of P16 protein were performed for 35 cases of breast cancer tissues,35 cases of breast cancer adjacent tissues,30 cases of breast fibroadenoma and 30 cases of normal breast tissues surgically removed in Department of General Surgery,First Affiliated Hospital of Shanxi Medical University.The biological data obtained were then analyzed using the statistical analysis toolkit（SPSS11.5）,and P≤0.05 was considered statistically significant.Results The positive rate of HPV16/l8 expression was 71% in breast cancer tissues,which reached the level of statistical difference compared with those in breast fibroadenoma and normal breast tissues（P≤0.05）.The expressions of HPVl6/18 in breast cancer tissues were positively associated with armpit lymph node metastasis（r= 0.152,P=0.021）,but not with menopause and tumor size.There was a negative correlation between the expressions of HPVl6/18 and P16 in breast cancer tissues（r=-0.662,P=0.000）.Conclusion HPV was shown to be highly expressed in breast cancer,which might be etiologically relevant in occurrence of breast cancer.Moreover,HPV infection was positively associated with armpit lymph node metastasis,and therefore could be a marker of progression and prognosis for breast cancer.HPV infection may also be negatively correlated with inactivation of P16,suggesting that interactions between the both may be involved in the occurrence and development of breast cancer.

关 键 词：乳腺肿瘤 乳头瘤病毒 人 蛋白质P16 

分 类 号：R737.9[医药卫生—肿瘤]