中和剂对稀酸蒸爆玉米秸秆酶解效果的影响  

Effects of neutralizing agents on enzymatic hydrolysis of corn stover pretreated by dilute acid-mediated steam explosion

作　　者：张红漫[1] 赵晶[1] 林增祥[2] 黄和[2] 

机构地区：[1]南京工业大学理学院,南京210009 [2]南京工业大学生物与制药工程学院,南京210009

出　　处：《生物加工过程》2011年第1期1-4,共4页Chinese Journal of Bioprocess Engineering

基　　金：国家自然科学基金资助项目(20876078);国家高技术研究发展计划(863计划)资助项目(2009AA02Z08);NSFC-广东联合基金项目(U0733001);中石化项目(207035)

摘　　要：以稀酸蒸爆的玉米秸秆为研究对象,考察直接水洗、Ca(OH)2、NaOH、氨水中和物料至pH 5,在固液比1∶10、酶添加量为每克纤维素14 U(滤纸酶活)的酶解条件下对纤维素转化率的影响。结果表明:水洗、Ca(OH)2、NaOH、氨水中和物料酶解72 h后,纤维素转化率分别为91.7%、80.7%、83.1%及81.7%。同时对影响纤维素酶解效率的各种因素进行了探讨。从综合成本及后续发酵过程考虑,用氨水中和稀酸蒸爆物料更适合于工业化生产。Corn stovers pretreated by dilute sulphuric acid-mediated steam explosion were neutralized to F,It 5 with Ca（ OH）2, NaOH and NH4OH, or alternatively washed with water to the same pH. The cellu- lose conversion rates by enzymatic hydrolysis under the conditions of solide liquid ratio of 1 ： 10 and cellulases loading of 14 U/g cellulose were investigated. The results showed that after enzymatic hydrolysis for 72 h, the cellulose conversion rates of the cooresponding samples were up to 91.7% , 80.7%o , 83.1% and 81.7% , respectively. Meanwhile, the effects of various factors on the efficency of ccllulase hysrolysis were also discussed. Considering the cost and following fermentation process, the neutralizing agent NH4OH was the choice for the industrialization production.

关 键 词：玉米秸秆 酶水解 中和 蒸爆 

分 类 号：Q556[生物学—生物化学]