芍药属牡丹组的系统学研究——基于RAPD分析  

Systematic studies on Paeonia sect. Moutan DC. based on RAPD analysis

作　　者：邹喻苹[1] 蔡美琳[1] 王子平 

机构地区：[1]中国科学院植物研究所系统与进化植物学开放研究实验室

出　　处：《植物分类学报》1999年第3期220-227,共8页Acta Phytotaxonomica Sinica

基　　金：中国科学院分类与区系学科特别支持项目

摘　　要：芍药属牡丹组（Ｐａｅｏｎｉａｓｅｃｔ．ＭｏｕｔａｎＤＣ）是落叶亚灌木，其野生类群为我国特有。长期以来不同学者根据形态性状对这个组中种的分类处理不断修正，不断有新种描述。我们采用ＲＡＰＤ标记分析了牡丹组种内与种间遗传关系。从１０个ＲＡＰＤ引物获得１２１个多态位点。用ＵＰＧＭＡ方法构建的树系图表明每个种的所有个体都各自聚为一支，种内的相似性系数为０．６０～０．９０，因此现有的７个种能很好地区分开来。Ｐ．ｄｅｌａｖａｙｉ与Ｐ．ｌｕｄｌｏｗｉ相似性系数为０．６０，聚为一支；Ｐ．ｊｉｓｈａｎｅｎｓｉｓ与Ｐ．ｒｏｃｋｉ、Ｐ．ｏｓｔｉ、Ｐ．ｑｉｕｉ以及Ｐ．ｄｅｃｏｍｐｏｓｉｔａ之间的相似性系数为０．４８，聚为一大支。这两支与肉质花盘亚组和革质花盘亚组相对应。这些结果与洪德元根据形态性状对该组所做的分类处理基本相符。我们认为ＲＡＰＤ技术用于牡丹基因组分析是灵敏而行之有效的工具。Plants in Paeonia sect.Moutan DC.,whose wild types are endemic to China, are deciduous subshrubs.Taxonomic treatments of most species in this section have long been in dispute. To address this question, both intraspecific and interspecific relationships of the species in this section were analyzed using RAPD markers. The dendrogram constructed by UPGMA showed that the accessions of the same species were always grouped together earlier than those of different species.The intraspecific similarity coefficients ranged from 0.60 to 0.90 , grouping precisely those species of the same subsection together. Hence,the seven species under question can be well distinguished from each other. The similarity coefficient between P.delavayi and P.ludlowii was 0.60, and they were clustered in a clade. The similarity coefficients between P. jishanensis and the three species P.rockii, P.ostii, P.qiui, and between P.jishanensis and P.decomposita were both 0.48. These five species were clustered in another clade. These two clades corresponded well to Subsect. Delavayanae and Subsect. Vaginatae. Our results support the taxonomic treatment of Sect. Moutan recently proposed by Hong (1998,1997).

关 键 词：随机扩增的多态性DNA 芍药属牡丹组 系统学 

分 类 号：Q949.762.3[生物学—植物学]