沙棘水溶性膳食纤维的提取及结构分析  

Extraction and structural analysis of sea buckthorn soluble dietary fiber

作　　者：黄鹏[1,2] 刘畅[1,2] 王珏[1] 王昌涛[1] 

机构地区：[1]北京工商大学植物资源研究开发北京市重点实验室,北京100048 [2]东北农业大学生命学院,哈尔滨150030

出　　处：《食品科技》2011年第2期203-206,211,共5页Food Science and Technology

基　　金：北京市科技新星项目(2008B08)

摘　　要：采用传统水提法提取沙棘水溶性膳食纤维,讨论固液比、浸提液pH、浸提时间和温度等条件对提取率的影响,通过红外光谱、液相和气相色谱等分析手段初步研究了沙棘水溶性膳食纤维的结构组成。结果表明:水提法提取最佳条件为水料比35、pH为7、浸提温度80℃、提取时间30min,在此条件下沙棘水溶性膳食纤维得率可达4.53%,平均分子量为2.065×105,单糖组成为葡萄糖、鼠李糖、阿拉伯糖、木糖、甘露糖、半乳糖。在沙棘SDF分子链中存在分支结构。Adopting the traditional water-extraction to extract sea buckthorn soluble dietary fiber（SDF）.Discuss some facts＇ impact on the extraction rate such as： water-feed ratio,extract pH value,extraction time and temperature conditions.Finally,the structures of SDF were preliminarily analyzed by means of FT-IR,UV-IR,LPC and GPC.The results show that the optimum extraction conditions is water feed ratio 35,pH value of 7,extraction temperature 80℃,extraction time of 30 min.Under these conditions,the sea buckthorn soluble dietary fiber gets up to 4.53%,with a molecular mass of 2.065×105.Sea buckthorn SDF is composed of glucose,rhamnose,arabinose,xylose,mannose and galactose,and have a branch structure in molecular chain.

关 键 词：沙棘 水溶性膳食纤维 提取工艺 结构分析 

分 类 号：R284.2[医药卫生—中药学]