GC-MS定量测定山豆根非生物碱部分成分含量  

Analysis of non-alkaloids from Radix Sophorae Tonkinensis by GC-MS

作　　者：祝路[1] 齐心[1] 高月求[2] 赵爱华[1] 贾伟[1] 

机构地区：[1]上海交通大学药学院,上海200240 [2]上海中医药大学附属曙光医院,上海200021

出　　处：《中国中药杂志》2011年第4期468-473,共6页China Journal of Chinese Materia Medica

基　　金：上海市中药现代化专项创新计划项目(07DZ19715)

摘　　要：目的:采用GC-MS定性、定量分析山豆根非生物碱中的部分化学物质组成和含量。方法:非生物碱部位样品经TMS衍生后,采用GC-MS法测定,通过NIST 2.0数据谱库检索,结合人工解析,鉴定各种化学成分。在此基础上,测定了各组分的含量。结果:从非生物碱部位共鉴定了24个化合物,其中定量测定了19个化合物的含量,占总量的34.72%,其中5个糖类成分占22.62%,9个有机酸占11.81%,其他5个成分占0.29%。结论:该法简便、快速、灵敏度高、重复性好,为定性、定量测定植物中复杂初生代谢产物提供了一种有效方法。Objective： To analyze the chemical compositions and their contents in non-alkaloids fraction from Radix Sophorae Tonkinensis. Method： The non-alkaloids from Radix Sophorae Tonkinensis were analyzed by gas chromatography/mass spectrometry after trimethylsilyl derivatization. The chemical compositions were identified by comparison of the authentic sample and NIST database ; the contents of 19 identified components were determined by linear regression equation. Result： 19 compounds constituting 34.72% of the non-alkaloids were identified. The major components were saccharides （5 compounds constituting 22.62% ）, organic acid （9 compounds constituting 11.81% ）, and others （5 compounds constituting 0.29% ）. Conclusion： The GC-MS is a simple, rapid and sensitive method to investigate the complex primary metabolites in plants.

关 键 词：山豆根 非生物碱 气相色谱-质谱联用 

分 类 号：R284[医药卫生—中药学]