高效液相色谱法测定烟草中有机酸  

HPLC Determination of Organic Acids in Tobacco

作　　者：李银科[1,2] 李海燕[1,3] 何顺琴[1,2] 薛景娇[1,2] 曹靖丽[1,2] 杨光宇[1] 胡秋芬[1,2] 

机构地区：[1]云南省烟草化学重点实验室,云南烟草科学研究院,昆明650106 [2]云南民族大学化学与生物技术学院,昆明650031 [3]云南省烟草质量监督检测站,昆明650106

出　　处：《理化检验（化学分册）》2011年第1期62-64,67,共4页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：云南省省院省校科技合作项目(2006YX17);中烟云南工业公司重大专项(2006B309)资助

摘　　要：提出了高效液相色谱法测定烟草中有机酸含量的方法。烟草样品经0.1 mol·L^-1氢氧化钠溶液高速匀浆提取,提取液通过装有MCI-GEL反相树脂固相萃取小柱净化,以ZORBAXStable Bound色谱柱（4.6 mm×150 mm,1.8μm）为固定相,0.01 mol·L^-1磷酸二氢钠溶液（pH2.98）和乙腈（98＋2）溶液为流动相梯度洗脱,用二极管阵列检测器于210 nm波长处检测。烟草中主要的有机酸均在12 min内达到基线分离,方法加标回收率在95.0%-103.0%之间,相对标准偏差（n=7）在1.8%-2.8%之间。A HPLC method for the determination of organic acids in tobacco was proposed.The sample was extracted with 0.1 mol·L^-1 NaOH solution by high-speed homogenization,and the extract obtained was purified on MCI-GEL reversed phase resin SPE column.The organic acids were separated by gradient elution with mixed solutions of 0.01 mol·L^-1 NaH2PO4 of pH 2.98 and acetonitrile（98＋2） in different ratios on ZORBAX Stable Bound column（4.6 mm×150 mm,1.8 μm）,and determined at the wavelength of 210 nm with diode array detector（DAD）.The main organic acids in tobacco were successfully separated within 12 min,giving values of recovery in the range of 95.0%-103.0% and RSD′s（n=7） in the range of 1.8%-2.8%.

关 键 词：高效液相色谱法 高速匀浆 有机酸 烟草 

分 类 号：O657.7[理学—分析化学]