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出 处:《中国实验方剂学杂志》2011年第5期83-86,共4页Chinese Journal of Experimental Traditional Medical Formulae
基 金:辽宁省教育厅项目(2009A498)
摘 要:目的:建立HPLC同时测定鼠掌老鹳草中没食子酸,原儿茶酸,槲皮素,山奈素,山奈素-3,7-O-α-二鼠李糖苷的含量测定方法。方法:采用RP-HPLC,Agilent TC-C18色谱柱(4.6 mm×250 mm,5μm),0.4%磷酸水溶液-甲醇为流动相梯度洗脱,流速0.8 mL·min-1,检测波长为切换波长,0~23 min,270 nm;23~55 min,370 nm,柱温30℃。结果:没食子酸,原儿茶酸,槲皮素,山奈素,山奈素-3,7-O-α-二鼠李糖苷的线性范围0.192~1.732 g·L-1(r=0.999 7),0.016~0.256 g·L-1(r=0.999 9),0.004~0.039 g·L-1(r=0.999 9),0.004~0.041 g·L-1(r=0.999 9),0.200 4~1.603 2 g·L-1(r=0.999 8),平均回收率(n=3)分别为99.93%(RSD 1.5%),98.27%(RSD 1.7%),100.07%(RSD 2.2%),99.92%(RSD 1.8%),99.63%(RSD1.0%)。结论:该含量测定的方法快速、准确、可靠、重复性好,可为药材的质量控制提供依据。Objective:To determine 5 active compounds in Geranium sibiricum,namely gallic acid,protocat-echuin acid,quercetin,kaempferol-7-O-rhamnoside and kaemferitrin.Method:The HPLC method was applied and the separation was performed on an Agilent TC-C18 analytical column(4.6 mm × 250 mm,5 μm).The mobile phase was methanol(A)-water containing 0.4% H3PO4(B) with gradient elution mode at the flow rate of 0.9 mL·min-1.The detection was set at 270 nm(0-23 min),370 nm(23-55 min),and the column temperature was 30 ℃.Result:The liner ranges of gallic acid,protocatechuin acid,quercetin,kaempferol-7-O-rhamnoside and kaemferitrin were 0.192-1.732 g·L-1(r = 0.999 7),0.016-0.256 g·L-1(r = 0.999 9),0.004-0.039 g·L-1(r = 0.999 9),0.004-0.041 g·L-1(r = 0.999 9),0.200 4-1.603 2 g·L-1(r = 0.999 8).The average recoveries(n = 3) were 99.93%(RSD 1.5%),97.27%(RSD 1.7%),100.07%(RSD 2.2%),99.92%(RSD 1.8%),99.63%(RSD 1.0%).Conclusion:The methord is simple and accurate with a good reproducibility and can be used as a quality control methord for G.sibiricum of different sources.
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