霍山石斛组织培养及快速克隆繁殖技术  

Tissue Culture and Rapid Cloning Propagation of Dendrobidium Huoshanness

作　　者：李蕤[1] 王琳[1] 陈群[1] 樊家荣[1] 

机构地区：[1]合肥大学生物与环境工程系,安徽合肥230022

出　　处：《药物生物技术》2011年第1期11-15,共5页Pharmaceutical Biotechnology

基　　金：安徽省高校自然科学基金资助项目(KJ2009B244Z)

摘　　要：为药用霍山石斛野生资源的保护和快速克隆繁殖,以霍山石斛幼茎为外植体,利用植物组织培养技术,成功培养了大量的霍山石斛幼苗。研究了培养温度、pH、添加物和激素配比等组织培养条件和调控因素对霍山石斛幼芽增殖的影响。结果表明:在MS为固体培养基,激素组合为0.3 mg/L NAA和0.5 mg/L 6-BA,温度25℃,蔗糖浓度30 g/L,香蕉泥为添加物,其浓度为150 g/L的优化培养条件下,石斛外植体生长情况最佳,最高出芽数达到96个。霍山石斛组培苗1年快速克隆繁殖系数可达4.812,移栽后植株成活率90%以上。To protect natural resource of medicinal plant Dendrobidium huoshanness and to realize rapid clonig propagation,the chit of Dendrobidium huoshanness was successfully cultured by plant tissue culture techniques using shoot tip of dendrobidium huoshanness as explants.The various conditions of plant tissue culture,such as different additives,temperature,pH value and various hormones ratio for Dendrobidium huoshanness were investigated.The experimental results showed that optimization conditions of plant tissue culture for Dendrobidium huoshanness is MS solid medium,0.3mg/L NAA,0.5mg/L 6-BA,temperature of 25 ℃,sucrose concentration 30g/L,and banana as additive（150g/L）.Under optimal conditions of plant tissue culture,the growth potential of Dendrobidium huoshanness is the highest and the sprouting amount reaches to 96.The coefficient of rapid cloning propagation for one year is up to 4.812.The survival rate of plantlets was over 90% after being transplanted.

关 键 词：霍山石斛 外植体 组织培养 快速克隆繁殖 

分 类 号：Q943.1[生物学—植物学]