Synthesis,DNA binding and cleavage studies of the copper(Ⅱ) complexes of PNA-cyclen conjugates  

作　　者：ZHANG Yu HUANG Yu ZHANG Ji ZHANG DaWei LIU JunLiang LIU Qiang LIN HongHui YU XiaoQi 

机构地区：[1]Key Laboratory of Green Chemistry and Technology, Ministry of Education College of Chemistry, Sichuan University, Chengdu 610064, China [2]Key Laboratory of Bio-resources and Eco-environment, Ministry of Education College of Life Sciences, Sichaan University, Chengdu 610064, China

出　　处：《Science China Chemistry》2011年第1期129-136,共8页中国科学（化学英文版）

基　　金：financially supported by the National Natural Science Foundation of China (20725206, 20732004 & 20902062);Program for Changjiang Scholars and Innovative Research Team in University, the Key Project of Chinese Ministry of Education in China and Scientific Fund of Sichuan Province for Outstanding Young Scientists

摘　　要：Two dinucleotide PNA-cyclen copper(II) complexes with α-PNA (P1) and classical PNA (P2) backbones were synthesized and characterized.The interactions between title complexes and DNA were investigated under physiological conditions.Fluorescence studies indicate that the binding ability of complex P1 to CT-DNA is as twice as that of P2.DNA melting experiments were also carried out and the results show that ΔTm caused by P1 is higher than that caused by P2.Agarose gel electrophoresis experiments demonstrate that P1 is an excellent chemical nuclease,which can cleavage plasmid DNA completely in 12 h.Two dinucleotide PNA-cyclen copper（II） complexes with α-PNA （P1） and classical PNA （P2） backbones were synthesized and characterized.The interactions between title complexes and DNA were investigated under physiological conditions.Fluorescence studies indicate that the binding ability of complex P1 to CT-DNA is as twice as that of P2.DNA melting experiments were also carried out and the results show that ΔTm caused by P1 is higher than that caused by P2.Agarose gel electrophoresis experiments demonstrate that P1 is an excellent chemical nuclease,which can cleavage plasmid DNA completely in 12 h.

关 键 词：PNA CYCLEN copper complexes DNA binding DNA cleavage 

分 类 号：Q523.03[生物学—生物化学] O614.121[理学—无机化学]