放射线诱发Lewis肺癌细胞株凋亡的研究  

Lewis Cell Apoptosis Induced by Radiation

作　　者：张燕[1] 苏长青[1] 陶晨[1] 

机构地区：[1]南京市第二医院 [2]解放军第八一医院

出　　处：《实用癌症杂志》1999年第3期174-177,共4页The Practical Journal of Cancer

摘　　要：对６０Ｃｏ照射的Ｌｅｗｉｓ细胞及其小鼠移植瘤进行电镜、ＤＮＡ电泳和流式细胞术观察，研究照射Ｌｅｗｉｓ细胞凋亡的时间、剂量效应关系，以及对小鼠移植瘤的影响。发现照射后Ｌｅｗｉｓ细胞出现的凋亡随照射剂量增加和照射后培养时间延长而升高；在移植瘤中持续出现的细胞凋亡与照射后培养时间的长短相关。可以认为放射线诱发Ｌｅｗｉｓ细胞凋亡的模型对肺癌的放疗有一定的参考意义；Ｌｅｗｉｓ细胞凋亡可能存在多种发生机制。Lewis cell line and its mouse transplanted tumors radiated by 0 60 Co were observed under electron microscopy,DNA gel electrophoresis and flow cytometry.Radiation induced Lewis cell apoptosis increased with the rise of radiation dose and the prolongation of cell culture time after radiation.Apoptosis appeared in mouse transplanted tumors was related to the culture time.The results suggested that the model of radiation induced Lewis cell apoptosis may play an important role in study of lung cancer radiotherapy.There are multiple mechanisms in Lewis cell apoptosis induced by radiation.

关 键 词：肺癌 放射诱导 细胞凋亡 

分 类 号：R734.205[医药卫生—肿瘤]