TopoⅡ α在乳腺癌中的表达及其临床意义  

Expression and clinical significance of Topo Ⅱ α in breast carcinoma

作　　者：何奇[1] 徐国萍[1] 王杰[1] 邵玉国[1] 

机构地区：[1]上海交通大学医学院附属国际和平妇幼保健院乳腺科,上海200030

出　　处：《中国癌症杂志》2011年第1期41-45,共5页China Oncology

摘　　要：背景与目的:DNA拓扑异构酶Ⅱα(topoisomeraseⅡα,TopoⅡα)是一个与恶性肿瘤增殖相关的基因,目前其对乳腺癌发生发展的影响已成为研究热点。本研究探讨TopoⅡα在乳腺良恶性疾病中的表达特点及其与乳腺癌临床病理特征的关系及其临床意义。方法:用免疫组化SP法检测了97例乳腺浸润性导管癌、32例导管内癌、25例导管上皮不典型增生和40例乳腺病中TopoⅡα蛋白的表达水平。结果:TopoⅡα在乳腺病组织、导管上皮不典型增生组织、导管内癌组织及浸润性导管癌组织中的阳性率分别为25%、28%、53.1%和53.6%,在乳腺上皮增生发展为癌的过程中,TopoⅡα表达水平逐渐增高(P<0.05);TopoⅡα在有淋巴结转移组表达阳性率为74.4%,显著高于无淋巴结转移组44.8%(P<0.01);CerbB-2阳性的乳腺癌TopoⅡα的阳性率为73.1%,CerbB-2阴性的乳腺癌TopoⅡα的阳性率为37.8%,两者相比,差异有统计学意义(P<0.01)。TopoⅡα表达与年龄、肿瘤的大小、组织学分级、临床分期、ER及PR无相关性(P>0.05)。结论:TopoⅡα表达增高对乳腺癌的发生和发展具有一定作用,TopoⅡα表达可能与乳腺癌的淋巴结转移有关,与CerbB-2高表达相关,可为乳腺癌的治疗效果提供必要的理论依据。Background and purpose： DNA Topoisomerase Ⅱα（lopoⅡα） is a gene involved in the proliferation of malignant tumors. Its influence in the development and progression of breast cancer caused increasing interest in its research. This study investigated the expression of TopoⅡα in benign and malignant breast diseases and its clinical significance in breast carcinoma. Methods： The expression of Topo Ⅱα was detected using immunohistochemistry in 97 infiltrating ductal carcinoma tissues, 32 intraductal carcinoma tissues, 25 atypical hyperplasia of ductal epithelium tissues and 40 mastopathia tissues. Results： The positive expression of Topo Ⅱα was found in 53.6% of infiltrating ductal carcinoma tissues, 53.1% of intraductal carcinoma tissues, 28% of atypical hyperplasia of ductal epithelium tissues, and 25% of mastopathia tissues. Statistically significant differences and positive correlations in TopoⅡα expression among the stages of disease progression from normal to invasive carcinoma were observed （P〈0.05）. The positive expression rate of TopoⅡα in breast carcinoma tissues with lymph node metastases was significantly higher than that with no lymph node metastases （P〈0.01）. Statistically significant differences in the presence of TopoⅡα （＋）/CerbB-2 （＋） 73.1% and Topo Ⅱα （＋）/CerbB-2 （-） 37.8% patterns were noted in the invasive carcinoma （P〈0.01）. No statistically significant differences in invasive carcinoma were found between TopoⅡα and age, tumor size, histological class, clinical stage, ER or PR （P〉0.05）. Conclusion： These results suggest that the increased expression of TopoⅡα may play an important role in the development and progression of the breast carcinoma. There may be an association between the expression of TopoⅡα and lymph node metastases as well as the high expression of CerbB-2.

关 键 词：乳腺癌 TopoⅡα 免疫组化 

分 类 号：R737.9[医药卫生—肿瘤]