左旋咪唑通过促进抗原呈递细胞MHC-Ⅱ表达而加重实验性自身免疫性脑脊髓炎  

Levamisole Aggravates Experimental Autoimmune Encephalomyelitis by Promoting the Expression of MHC-Ⅱ of Antigen Presenting Cells

作　　者：王赵伟[1] 郑荣远[1] 林福虹[1] 王沛[1] 张正学[1] 

机构地区：[1]温州医学院附属第一医院神经科温州医学院实验神经生物学研究所,325000

出　　处：《中国临床神经科学》2011年第1期1-4,共4页Chinese Journal of Clinical Neurosciences

基　　金：浙江省自然科学基金项目(编号:Y204133);国家自然科学基金项目(编号:81070960)

摘　　要：目的:探讨左旋咪唑加重大鼠实验性自身免疫性脑脊髓炎(EAE)是否通过上调抗原呈递细胞(APC)主要组织相容性复合体Ⅱ类抗原(MHC-Ⅱ)表达而实现的。方法:SD大鼠28只随机分为完全弗氏佐剂(CFA)组(n=8),EAE组(n=10),左旋咪唑组(n=10)。用豚鼠脊髓匀浆和CFA的混匀乳剂免疫SD大鼠制作EAE模型。在造模前3d至造模后7d腹腔注射左旋咪唑10mg·kg-1(左旋咪唑组)。分析各组行为学变化及神经组织病理学改变,用流式细胞术检测脾脏APC表面分子MHC-Ⅱ的表达。结果:左旋咪唑加速了大鼠EAE的病程,加重了神经缺损症状与中枢神经病理学损害;左旋咪唑上调了脾脏APC表面分子MHC-Ⅱ的表达,且与EAE神经缺损症状程度呈正相关。结论:左旋咪唑上调了APC表面分子MHC-Ⅱ的表达可能为左旋咪唑促发EAE的作用机制。Aim：To study the relationship between the effect of levamisole（LMS） aggravating experimental autoimmune encephalomyelitis and its function of regulating MHC-Ⅱ（major histocompatibility complex class Ⅱ,MHC-Ⅱ）expression on splenic antigen presenting cells. Methods：Rats were intraperitoneal injected with LMS at a dose of 10 mg·g-1 from 3 days before EAE model made to 7 days after EAE model made. The effect of levamisole on EAE was evaluated both by clinical scores of EAE and neuropathology disorder. The expressions of MHC-Ⅱ in splenic antigen presenting cells were compared by flow cytometry. Results：LMS aggravates experimental autoimmune encephalomyelitis with shorted latency of EAE and neuroethology disorder was enhanced. At the same time,LMS up-regulated the expression of MHC-Ⅱ in splenic antigen presenting cells and was positively correlative with the severity of EAE. Conclusion：Levamisole aggravates experimental autoimmune encephalomyelitis by promoting the expression of MHC-Ⅱ of antigen presenting cells.

关 键 词：左旋咪唑 实验性自身免疫性脑脊髓炎 抗原呈递细胞 主要组织相容性复合体Ⅱ类抗原 

分 类 号：R74[医药卫生—神经病学与精神病学]