实验性自身免疫性脑脊髓炎小鼠脑组织和脊髓中脑型肌酸激酶、钙泵和钙中性蛋白酶的变化  

Changes of BB Isoenzyme of Creatine Kinase,CaATPase and Calpain in Experimental Autoimmune Encephalomyelitis Mouse Brain and Spinal Cord

作　　者：王沛[1] 郑荣远[1] 林福虹[1] 王赵伟[1] 厉芳[1] 张正学[1] 

机构地区：[1]温州医学院附属第一医院神经内科,325000

出　　处：《中国临床神经科学》2011年第1期9-15,共7页Chinese Journal of Clinical Neurosciences

摘　　要：目的:观察实验性自身免疫性脑脊髓炎(EAE)小鼠模型脑组织和脊髓中脑型肌酸激酶(CK-BB)、钙泵(CaATPase)和钙中性蛋白酶(calpain)的变化。方法:C57BL/6小鼠随机分为:EAE组(n=9),用髓鞘少突胶质细胞糖蛋白35-55多肽(MOG35-55)及完全弗氏佐剂混合抗原乳剂免疫小鼠;对照组(n=5),注射生理盐水。用MOG35-55诱导C57BL/6小鼠,建立(EAE)动物模型(即多发性硬化模型)。观察记录EAE小鼠行为学变化;采用苏木精-伊红染色、LFB髓鞘染色,酶标仪和分光光度计法检测发病高峰期(免疫后第19天)的中枢神经组织病理学变化、CK-BB,CaATPase和calpain酶活性的改变。结果:EAE组与对照组相比:①日均神经功能评分和日累积评分增加(P<0.01);②苏木精-伊红染色:中枢炎症细胞浸润明显(P<0.05);③LFB染色:髓鞘脱失较多;④CK-BB和CaATPase活性降低,calpain活性增加(P<0.05);⑤EAE组小鼠(只)日均累积神经功能评分与CK-BB和CaATPase活性呈负相关(P<0.05);⑥EAE组小鼠(只)日均累积神经功能评分与calpain酶活性呈正相关(P<0.05)。结论:EAE高峰期中枢CK-BB和CaATPase活性降低、calpain活性增高是EAE发生发展的后果,提示EAE时中枢能量代谢和CaATPase、calpain的病理性损害。Aim：To investigate the changes of BB isoenzyme of creatine kinase（CK-BB）,CaATPase and calpain in experimental autoimmune encephalomyelitis（EAE） mouse brain and spinal cord. Methods：C57BL/6 mice were induced into the models of EAE with multiple sclerosis by myelin oligodendrocyte glycoprotein 35-55 （MOG35-55） peptides. Behavioral changes of the EAE mice were observed and recorded. With HE staining,LFB myelin staining,the changes of the central nervous tissues,CK-BB,CaATPase and calpain activity were assayed in the peak incidence by using microplate reader and spectrophotometer （19 days after immunization）. Results：Compared with the control group,the results of the EAE group were as follows：①Mean daily clinical scores and cumulative scores were increased（P0.01）. ②HE staining：Central inflammatory cell infiltration became obvious（P0.05）. ③LFB stain：Myelinoclasis became obvious. ④Enzyme activity：The activities of CK-BB and CaATPase decreased while calpain activity was increased（P0.05）. ⑤Cumulative average daily scores of each EAE mouse had negative correlation with the activities of CK-BB and CaATPase（P0.05）. ⑥ Cumulative average daily scores of each EAE mouse had positive correlation with the calpain activity（P0.05）. Conclusion：The decrease of acitivities of central CK-BB and CaATPase and the activity increase of calpain in the peak were the consequences of EAE occurrence,suggesting EAE occurrence involves the central energy metabolism and CaATPase,calpain pathological damage.

关 键 词：多发性硬化 实验性自身免疫性脑脊髓炎 脑型肌酸激酶 钙泵 钙中性蛋白酶 小鼠 

分 类 号：R744.51[医药卫生—神经病学与精神病学]