硫酸镍对SD大鼠晶状体损伤的实验研究  

Influence of nickel sulphate on lens in SD rat

作　　者：史才财[1] 张兴儒[1] 周欢明[1] 李青松[1] 项敏泓[1] 唐剑敏[2] 张隆敏[1] 张隆[3] 

机构地区：[1]上海中医药大学附属普陀医院眼科,200062 [2]上海中医药大学附属普陀医院病理科,200062 [3]上海中医药大学附属普陀医院中心实验室,200062

出　　处：《中华实验眼科杂志》2011年第2期135-138,共4页Chinese Journal Of Experimental Ophthalmology

摘　　要：背景流行病学调查表明，镍矿区人群中自内障的发病率高于其他人群，硫酸镍对人晶状体上皮细胞（LECs）的代谢和增生有抑制作用，但目前镍对晶状体损伤机制的研究较少。目的探讨硫酸镍对sD大鼠晶状体的损伤情况。方法出生7～14d的SPF级sD大鼠45只随机分为空白对照组、皮下注射组、腹腔注射组。腹腔注射组、皮下注射组分别腹腔注射或皮下注射2g／L的硫酸镍溶液10mg／kg，连续给药45d；空白对照组不用药。给药后每2周裂隙灯下观察sD大鼠晶状体的变化，给药后45d参考LOCSⅡ、LOCSⅢ的评价标准对晶状体的前囊、核区、皮质和后囊进行混浊指数评分，评价sD大鼠晶状体的混浊程度。45d后处死大鼠，取下完整晶状体行组织学观察。结果与空白对照组比较，皮下注射组大鼠晶状体前囊区混浊指数评分较高，差异有统计学意义（t=14．311，P〈0．05），腹腔注射组大鼠晶状体前囊区混浊指数评分虽高于空白对照组，但差异无统计学意义（t=4．355，P〉0．05）。皮下注射组和腹腔注射组大鼠晶状体后囊混浊指数评分高于空白对照组，差异均有统计学意义（t=9．316，P=0．004；t=7．464，P=0．009）。皮下注射组和腹腔注射组大鼠晶状体前囊＋后囊晶状体混浊指数评分高于空白对照组，差异均有统计学意义（t=23．387，P=0．000；t=10．533，P=0．002）。各组大鼠晶状体皮质区和核区混浊指数评分比较差异均无统计学意义（P〉0．05）。皮下注射组、腹腔注射组大鼠晶状体混浊指数总评分高于空白对照组，差异均有统计学意义（t=12．358，P=0．001；t=10．188，P=0．003）；皮下注射组晶状体混浊的总评分高于腹腔注射组，但差异无统计学意义（t=0．527，P〉0．05）。皮下注射组和腹腔注射组大鼠晶状体胶原纤维变性均比空白对照组严重，皮下注射组大鼠的晶状体胶�Background Researches showed that the incidence rate of cataract is high in the nickel mining area. Nickel sulphate can apparently inhibit the metabolism and proliferation of human lens epithelium cells. But the study on the injury mechanism of nickel on lens is still seldom. Objective Present study was to investigate the effect of nickel sulphate on the lens of SD rats. Methods Forty-five SPF SD rats aged from 7 to 14 days were grouped randomly into subcutaneous injection group,intraperitoneal injection group and blank group. Nickel sulphate of 2 g/L （ 10 mg/kg） was subcutaneously or intraperitonealy injected for 45 days. The opacity of rat lens was examined under the slit lamp at two-week interval and scored based on the criteria of LOCS Ⅱand LOCS Ⅲ. The rats were sacrificed in 45 days after experiment and the lens were obtained for the pathological examination. Result The mean score of the anterior subcapsule opacity of rat lens was obviously higher in subcutaneous injection group compared with blank control group with a significant difference between them （t = 14. 311, P〈0.05 ）, but no significant difference in the anterior subcapsule opacity between intraperitoneal injection group and blank control group （t=4. 355 ,P〉0. 05）. The score of posterior subcapsule opacity of lens were evidently higher in both subcutaneous injection group and intraperitoneal injection group than the blank control group （t = 9. 316 ,P = 0. 004 ;t = 7.464 ,P = 0. 009） , so was the mean score of the anterior ＋posterior subcapsule opacities （t=23. 387, P = 0. 000;t = 10. 533, P = 0.002） and the total score of rat lens opacity （t= 12. 358,P=0.001;t= 10.188,P=0.003）. No significant differences were found in cortex opacity score and nuclear opacity score among three groups （P 〉 0. 05 ）. Histopathology examination revealed that the degeneration of lens collagen protein was more serious in subcutaneous injection group and intraperitoneal injection group than the blank control group,and the injury degr

关 键 词：硫酸镍 晶状体 白内障 

分 类 号：R77[医药卫生—眼科]