普那霉素生物合成相关基因Spr1的功能  

The Role of Spr1 Gene of Streptomyces pristinaespiralis in Pristinamycin Biosynthesis

作　　者：尹华立[1,2] 金志华[1,2] 金庆超[2] 

机构地区：[1]浙江大学化学工程与生物工程系,杭州310027 [2]浙江大学宁波理工学院生物与化学工程分院,宁波315100

出　　处：《中国生物化学与分子生物学报》2011年第2期154-160,共7页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家自然科学基金项目(No.20976161);浙江省自然科学基金项目(No.Y4100123);宁波市自然科学基金(No.2009A610149和No.2010A610023)~~

摘　　要：以与普那霉素生物合成密切相关的新基因Afsk-like为探针,从始旋链霉菌F618基因组文库中筛选得到含有约8 kb的DNA片段.经测序分析表明,其上含有1个具有1 146个核苷酸的完整可阅读框,该基因被命名为Spr1(HQ450023),推测其编码1个含381个氨基酸的蛋白质产物.经Blastp程序进行分析得知,该基因编码产物与谷氨酰胺合成酶有一定的同源性.经基因中断实验研究表明,该基因可能与普那霉素生物合成正相关.The cosmid library from the strain of pristinamycin-producing Streptomyces pristinaespiralis F618 was screened using a probe of partial Afsk-like gene that involved in pristinamycin biosynthsis,and an approximate 8 kb DNA fragment was obtained.The nucleotide sequence analysis indicated that a 1146 bp DNA fragment contained one complete open reading frame（ORF） coding for Spr1.The predicted protein was homologous to glutamine synthetase as shown by the Blastp program.To investigate the function of Spr1 gene（HQ450023）,we have isolated the Spr1 mutants,and then the fermentation culture of mutants and S.pristinaespiralis F618 were examined by HPLC.A positive correlation between the product of Spr1 gene and the pristinamycin biosynthesis was observed.

关 键 词：始旋链霉菌 普那霉素 基因中断 生物合成 谷氨酰胺合成酶 

分 类 号：Q78[生物学—分子生物学]