CDH12基因siRNA对涎腺腺样囊性癌细胞侵袭力的影响  

Role of CDH12 siRNA on invasiveness of salivary adenoid cystic carcinoma cells

作　　者：苏柏华[1] 王锦锋[1] 佘林[1] 郑斐斐[1] 丁林灿[1] 卢友光[1] 

机构地区：[1]福建医科大学附属口腔医院预防科,福州350002

出　　处：《重庆医科大学学报》2011年第1期11-15,共5页Journal of Chongqing Medical University

基　　金：福建省科技厅科技计划重点项目(编号:2008Y0041)

摘　　要：目的:探讨钙粘素12(Cadherin12,CDH12)对涎腺腺样囊性癌(Salivary adenoid cystic carcinoma,SACC)细胞侵袭和转移能力的影响。方法:以人涎腺腺样囊性癌高转移细胞株(Salivary adenoid cystic carcinoma cell line with high metastatic ability,SACC-M)为研究对象,利用CDH12小分子干扰RNA(Small interfering RNAs,iRNA)对CDH12基因进行沉默,Western blot检测转染前后CDH12的表达变化,Cell Counting Kit-8法检测转染前后细胞的生长速度,体外侵袭实验比较细胞侵袭能力变化,体外迁移运动实验比较细胞运动能力的改变。结果:CDH12 siRNA明显下调CDH12基因的表达。CDH12表达下调后,SACC-M细胞体外侵袭能力显著降低,体外迁移运动能力明显降低。结论:CDH12明显地促进了SACC细胞的体外侵袭和迁移运动,提示CDH12可能在SACC的恶性进展中起着重要作用。Objective：To study the effect of CDH12 on invasion and migration of human salivary adenoid cystic carcinoma cells in vitro.Methods：The highly metastatic human salivary adenoid cystic carcinoma cell line SACC-M was transfected by CDH12 siRNA to silence CDH12 gene.The protein expression of CDH12,before and after siRNA transfection,was examined by Western blot.Cell growth rate was determined with Cell Counting Kit-8.At the same time,the invasive capability and migration capability were evaluated by tumour invasion assay and migration assay,respectively.Results CDH12 siRNA transfection specifically down-regulated the CDH12 protein in SACC-M cells.The downregulation of CDH12 supressed the invasive capability and migration capability of SACC-M cells.Conclusion：CDH12 obviously promotes the invasion and migration of SACC cells in vitro.These results suggest that CDH12 may play an important role in the malignant progression in salivary adenoid cystic carcinoma.

关 键 词：小分子干扰RNA 钙粘素12 腺样囊性癌 侵袭 

分 类 号：R739.870.22[医药卫生—肿瘤]