高脂诱导的NAFLD大鼠模型血清IL-6、TNF-α等的变化  

Changes of plasma Interleukin-6 and TNF-α in the development of nonalcoholic fatty liver rats induced by high fat diet

作　　者：敬晓琴[1] 袁喆[1] 

机构地区：[1]重庆医科大学附属第一医院感染科重庆市传染病寄生虫病学重点实验室,重庆400016

出　　处：《重庆医科大学学报》2011年第1期31-34,共4页Journal of Chongqing Medical University

基　　金：重庆市卫生局课题(编号:2003-31)

摘　　要：目的:白细胞介素-6(Interleukin-6I,L-6)和肿瘤坏死因子(Tumor necrosis factor-α,TNF-α)在非酒精性脂肪肝病(Nonalcoholic fatty liver disease,NAFLD)的形成中具有重要作用。我们探讨在大鼠NAFLD发生发展过程中IL-6血清浓度的变化及与TNF-α、胰岛素抵抗(Insulin resistance,IR)的关系,从而更好地了解非酒精性脂肪肝病的发病机理。方法:48只雄性SD大鼠随机分为正常对照组和NAFLD模型组,正常对照组采用普通饲料喂养,NAFLD模型组采用高脂饲料喂养,并于第2、4、6、10周末分批处死各组大鼠6只,并检测其血清ALT、AST、TG、TC、空腹血糖(Fasting blood glucose,FBG)、空腹胰岛素(Fastinginsulin,FINS)I、L-6、TNF-α等指标,计算肝指数及胰岛素抵抗指数(Insulin resistance index,IRI),观察肝脏病理变化。结果:模型组大鼠血清ALT、AST、TG、TC、FINSI、RII、L-6、TNF-α均较对照组升高,其中TNF-α自第4周起高于对照组(P<0.05)I,L-6第6周起高于对照组(P<0.05)I,L-6与TNF-α(r=0.931,P<0.05)I、RI(r=0.946,P<0.05)呈高度正相关。结论:脂肪因子IL-6与TNF-αI、RI密切相关,与NAFLD的形成发展有重要关联。Objective：IL-6 and TNF-α play critical roles in the development of nonalcoholic fatty liver disease（NAFLD）.We thus investigated the changes of plasma Interleukin-6（IL-6） and TNF-α in the nonalcoholic fatty liver of rats and their correlation.This will provide new insight into the pathology of NAFLD.Methods：Forty-eight male SD rats were divided in two groups randomly.The NAFLD was induced in male SD rats by feeding the high-lipid nutrition,whereas,the control group was fed with low-fat nutrition.All rats were sacrificed at the end of 2,4,6,and 10 weeks.The levels of ALT,AST,TG,TC,FBG,FINS,IL-6 and TNF-α in the sera of rats were measured.The liver index and IRI were counted and the pathologic changes of liver were recorded during experiments.Results：Compared with control group,the levels of ALT,AST,TC,TG,FINS,IRI,TNF-α and IL-6 in the sera of NAFLD group all increased significantly（P0.01）.The levels of TNF-α in NAFLD group started to increase significantly at week 4（P0.05）,while the levels of IL-6 began to elevate from week 6（P0.05）.From week 2 to week 10,IL-6 was highly positively related to TNF-α（r = 0.931,P0.05） and IRI（r = 0.946,P0.05）.Conclusion：The levels of IL-6 were highly correlated with TNF-α and IRI in the sera of NAFLD mouse models,which indicates that both IL-6 and TNF-α are crucial in the development of NAFLD.

关 键 词：非酒精性脂肪肝 脂肪因子 白细胞介素-6 

分 类 号：R575.5[医药卫生—消化系统]