不同表面修饰的Fe_3O_4纳米颗粒在体肿瘤成像研究  

In vivo MR imaging of tumor by using Fe_3O_4 nanoparticles bearing with different surface ligand as contrast agent

作　　者：乔瑞瑞[1] 刘侃[2] 贾兵[3] 曾剑峰[1] 欧阳汉[2] 王凡[3] 高明远[1] 

机构地区：[1]中国科学院化学研究所,北京100190 [2]中国医学科学院北京协和医学院肿瘤医院,北京100021 [3]北京大学医学同位素研究中心,北京100191

出　　处：《东南大学学报（医学版）》2011年第1期63-66,共4页Journal of Southeast University(Medical Science Edition)

基　　金：科技部国家重大科学研究计划资助项目(2011CB935800);国家自然科学基金资助项目(21003135)

摘　　要：目的:比较两种不同表面修饰的Fe3O4纳米颗粒作为肿瘤探针进行在体磁共振成像(MRI)的区别。方法:采用两种不同表面修饰的Fe3O4作为磁共振造影剂,并利用其表面羧基与具有靶向识别肿瘤表面整合素受体(Integrinαvβ3)的c(RGDyK)多肽进行耦联,制备出具有肿瘤靶向性的磁共振分子探针。以荷人脑胶质瘤(U-87 MG)裸鼠为动物模型,进行体内MRI研究。结果与结论:两种纳米颗粒均能够产生明显的T2造影效果,表面为聚乙二醇及油胺共同修饰的纳米颗粒的最佳成像时间为注射药物后8 h,而只有聚乙二醇修饰的纳米颗粒的最佳成像时间为注射药物后4 h,导致两种纳米颗粒在成像时达到最佳成像效果的时间不同的原因在于其表面电荷的不同。Objective： To compare the contrast effect for MR imaging of tumor by using two types of Fe3O4 nanoparticles bearing with different surface ligands as tumor-targeting contrast agent.Methods： The tumor-targeting peptide c（RGDyK） was conjugated with Fe3O4 nanoparticles bearing with carboxylic groups by the conjugation reaction mediated by EDC and sulfo-NHS.Then,the conjugates were applied to in vivo detecting tumors in BALB/c nude mice bearing U-87 MG human glioma cancer xenografts.Results and Conclusion： The two different types of Fe3O4 nanoparticles both can obtain obvious contrast effect,however,the best contrast images had to be acquired at different time.For particles bearing with PEG and oleyamine,the best contrast images can be acquired at 8 h after injection,while for particles bearing with PEG,it was 4 h after injection,which may be affected by the different surface charge of the Fe3O4 nanoparticles.

关 键 词：FE3O4纳米颗粒 分子成像 磁共振成像 精氨酸-甘氨酸-天冬氨酸肽 

分 类 号：R730.49[医药卫生—肿瘤] R319[医药卫生—临床医学]