原子力显微镜下几种癌细胞形态特征的共性分析  

Analysis of the common morphological characteristics of cancerous cells using atomic force microscope

作　　者：舒清明[1] 李月月[1] 朱茗[2] 张新武[1] 王晓东[1] 陈辉[1] 纪小龙[1] 

机构地区：[1]武警总医院病理科,北京100039 [2]武警广东省总队医院外四科,广东广州510515

出　　处：《南方医科大学学报》2011年第2期205-209,共5页Journal of Southern Medical University

基　　金：国家自然科学基金(30471934)~~

摘　　要：目的运用原子力显微镜(AFM)观察活体不同类型肿瘤细胞膜表面超微结构,并分析它们的共同特点。方法选取正常肝细胞及肝癌细胞、宫颈鳞状细胞及鳞癌细胞、乳腺导管上皮细胞及乳腺癌细胞各60例,利用AFM扫描标本,将细胞膜表面扫描图像数据用分析软件进行分析。结果正常宫颈鳞状上皮从表层细胞到中层细胞、底层细胞再到HPV感染细胞、CIN2-3级细胞及宫颈癌细胞的细胞膜表面平均粗糙度逐渐增大(P<0.05)。正常肝细胞组、乙型肝炎后肝硬化细胞组、肝细胞性肝癌细胞组细胞膜表面平均粗糙度数值逐渐增大(P<0.05)。正常乳腺组、乳腺增生症组、乳腺癌组细胞膜表面平均粗糙度也具有逐渐增大的趋势(P<0.05)。结论细胞种类不同,细胞膜表面结构不同。正常细胞膜与肿瘤细胞膜在形态结构上存在显著差别,特别是正常细胞膜与同种细胞来源的肿瘤细胞膜相比能明确判断出细胞是否恶变,这为临床病理诊断和鉴别诊断提供了可靠的依据。Objective To observe the surface ultrastructure of different tumor cells in vivo using atomic force microscope（AFM） and analyze their common characteristics.Methods We selected 60 specimens of each of normal liver cells,liver cancer,cervical squamous cells,cervical cancer cells,ductal epithelial cells and breast cancer cells for scanning using AFM.The cell surface scan images were analyzed using image analysis software to identify their common morphological features.Results From normal cervical squamous epithelial cells,intermediate cells,and basal cells to HPV-infected cells,CIN2-3 cells and cervical cancer cells,the membrane surface roughness became gradually increased（P0.05）.Similarly,the surface roughness increased significantly in the order of normal liver cells,hepatitis B cirrhosis liver cells,and hepatocellular carcinoma cells（P0.05）.The average surface roughness also tended to increase from normal mammary gland cells to mammary gland hyperplasia cells and breast cancer cells（P0.05）.Conclusion Normal cells and tumor cells show different cell membrane morphologies,and such morphological features provide a reliable basis for clinical pathological diagnosis and differential diagnosis of malignancies.

关 键 词：原子力显微镜 活体 癌细胞 膜蛋白 

分 类 号：R730.4[医药卫生—肿瘤]