早期肝癌血清蛋白质组标准模式图谱的建立及应用  

Establishment and application of standard serum proteomic pattern for diagnosis of early liver cancer

作　　者：陈仁[1] 陈小苹[1] 林秋雄[2] 林炳亮[3] 曹会娟[3] 

机构地区：[1]广东省医学科学院/广东省人民医院感染科,广东广州510080 [2]广东省医学科学院/广东省人民医院医学实验中心,广东广州510080 [3]中山大学附属第三医院感染科,广东广州510630

出　　处：《南方医科大学学报》2011年第2期341-343,共3页Journal of Southern Medical University

基　　金：广东省科技计划项目(2007B03070310)

摘　　要：目的构建早期肝癌血清蛋白质组标准模式图谱,初步探讨其在肝癌早期诊断中的临床意义。方法 (1)收集正常人、早期肝癌患者血清各12例,分别等量混匀为两组样本,去掉血清中的白蛋白和IgG。取血清各300μg与水化液充分混合,双向凝胶电泳,实验重复3次,银染,图像分析,筛选差异表达的蛋白点,构建早期肝癌血清蛋白质组标准模式图谱。(2)应用该模式图谱盲法对13例慢性肝脏疾病患者和10名健康人进行验证。结果筛选出两组间差异表达的蛋白点共38个,对表达量差异5倍以上的11个蛋白点进行分析,构建早期肝癌血清蛋白质组标准模式图谱。盲法分析示敏感性为92%,特异性90%。结论早期肝癌患者血清2-DE蛋白质图谱与健康人有一定差异,其差异表达的蛋白质及其特定组合构成有望成为早期肝癌诊断与判定预后的模式图谱。Objective To establish standard serum proteomic pattern for early liver cancer and explore its value in early liver cancer diagnosis.Methods The serum samples were obtained from 12 healthy subjects and 12 patients with early liver cancer.After removal of serum albumin and IgG,300 μg serum sample was mixed with the hydrating solution for two-dimensional gel electrophoresis.Each experiment was repeated for 3 times.With silver staining and image analysis,the differentially expressed proteins were identified to establish the standard proteomic pattern for early liver cancer.The proteomics pattern was then tested in the diagnosis of 13 cases of chronic liver disease and 10 healthy individuals in a double-blind manner.Results Thirty-eight differentially expressed protein spots were identified.Eleven protein spots with differential expressions by over 5 folds were analyzed to establish the standard proteomics pattern for early liver cancer,which showed a sensitivity of 92% and specificity of 90% in early diagnosis of liver cancer.Conclusion The standard serum 2-DE proteomic pattern for early liver cancer shows distinct differences from that of healthy subjects.The differentially expressed proteins and their specific combination pattern may have the potential for diagnosis and prognostic evaluation of early liver cancer.

关 键 词：早期肝癌 血清蛋白质组 模式图谱 

分 类 号：R735.7[医药卫生—肿瘤]