狂犬病病毒SRV-9株对小鼠脑神经原代细胞的感染及毒力观察  

Observation on infection and virulence of rabies virus SRV-9 strain on murine primary neuron cells

作　　者：胡乐鹏[1,2] 夏志平[1,2] 徐翠平[2] 刘存霞[3] 李志萍[3] 李勇[3] 刘雯[3] 袁子国[4] 张宁[3] 王利民[1] 

机构地区：[1]吉林农业大学动物科技学院,吉林长春130118 [2]军事医学科学院军事兽医研究所吉林省人兽共患病预防与控制重点实验室,吉林长春130122 [3]吉林大学畜牧兽医学院,吉林长春130062 [4]华南农业大学兽医学院,广东广州510642

出　　处：《中国兽医科学》2011年第1期5-8,共4页Chinese Veterinary Science

基　　金：农业公益性行业项目(200803014)

摘　　要：为建立一种狂犬病病毒(RV)感染原代神经细胞的体外培养方法,分离培养了新生小鼠脑神经原代细胞,并进行了RV感染试验,利用免疫荧光技术和免疫细胞化学试验检测了神经细胞中的RV及其对被感染细胞的影响。结果显示,RV可感染分离的原代神经细胞,并可以检测到针对RV的特异性荧光。表明,建立了可培养RV的原代神经细胞的制备方法,并且能够观察到原代神经细胞适应RV感染时的毒力变化。To establish a method used for infecting primary neuron cells by rabies virus in vitro and made some observations on toxicity and adaptability after infection,primary neuron cells were isolated and cultured from newborn mice which were infected with rabies virus SRV-9 strain later,and the virus was detected and the influence of the virus on the primary neuron cells was analyzed by the immune fluorescence technology and the immunocytochemistry tests.In result,the murine primary neuron cells were successfully infected by rabies virus and the specific fluorescence was detected.These results showed that the method for preparation of primary neuron cells suitable for culturing rabies virus was successfully developed and its toxicity change can be observed when murine primary neuron cells adapt to the infection of the virus.

关 键 词：狂犬病病毒 神经细胞 原代培养 免疫荧光 

分 类 号：S852.659.5[农业科学—基础兽医学]