酸蚀时间对牙本质Ⅰ型胶原降解的影响  

Effects of acid etching time on the degradation of type Ⅰ collagen in dentin

作　　者：胡琳[1] 肖玉鸿[2] 黄鹂[1] 孙翔[1] 张凌[1] 陈吉华[1] 

机构地区：[1]第四军医大学口腔医学院修复科,西安710032 [2]成都军区昆明总医院口腔科

出　　处：《中华口腔医学杂志》2011年第2期84-88,共5页Chinese Journal of Stomatology

基　　金：基金项目：国家自然科学基金（30973356、30901695）

摘　　要：目的 评价37%磷酸处理牙本质不同时间对牙本质Ⅰ型胶原降解的影响,以期为临床牙本质粘接操作提供实验依据.方法 37%磷酸酸蚀处理牙本质片0（空白对照组）、10、15、30、60 s,酶联免疫吸附法定量测定Ⅰ型胶原的降解量,场发射扫描电镜（field emission in-lens scanning electron microscope,FEISEM）观察胶原纤维的形态学变化.结果 酸蚀处理60 s时Ⅰ型胶原的降解量最多,为4.86（1.55）mg/g 其次为30 s组,为2.76（0.87）mg/g 再次为15 s组,为1.93（0.88）mg/g 10 s组胶原降解量较少,为0.95（0.38）mg/g 空白对照组胶原的降解量最少,为0.06（0.03）mg/g.两两比较显示,各组间差异均有统计学意义（P＜0.005）.FEISEM显示,37%磷酸处理牙本质表面10 s,尽管已清除了玷污层,但牙本质小管口以及胶原纤维仍覆盖颗粒样物质.酸蚀15 s牙本质小管口开放,暴露清晰.酸蚀30 s暴露的胶原纤维表面比15 s组光滑,球状附着物减少 酸蚀60 s牙本质小管内主纤维数最减少,管内结构塌陷,次级纤维数量增加,存在纤维断裂的征象.结论 在实验时间0～60 s内,酸蚀15 s即可达到酸蚀目的 ,延长酸蚀时间,可引起更多的胶原纤维变性降解.Objective To evaluate the effects of acid etching time on the degradation of type Ⅰcollagen in dentin. Methods Dentin was conditioned with 37% phosphoric acid for 10, 15, 30 and 60 s.There was no treatment for the control group. Quantity of collagen degradation in each group was determined by enzyme linked immunosorbent assay. Observations were carried out by means of a field emission in-lens scanning electron microscope （FEISEM）. Results Samples conditioned with 37% phosphoric acid for 60 s showed the most degradation of collagen, which was 4. 86 （1.55） mg/g, followed by 30 s group and 15 s group, which were 2.76（0.87） mg/g and 1.93（0.88） mg/g, respectively. Group of 10 s was 0.95（0. 38） mg/g. The control group showed the least degradation of 0. 06（0. 03） mg/g. Significant differences in collagen degradation were found among groupo （P 〈 0. 005）. Smear layer were removed well but tubular orifices and collagen fibrils were covered by particles after dentin being etched with 37% phosphoric acid for 10 Objective To evaluate the effects of acid etching time on the degradation of type Ⅰcollagen in dentin. Methods Dentin was conditioned with 37% phosphoric acid for 10, 15, 30 and 60 s.There was no treatment for the control group. Quantity of collagen degradation in each group was determined by enzyme linked immunosorbent assay. Observations were carried out by means of a field emission in-lens scanning electron microscope （FEISEM）. Results Samples conditioned with 37% phosphoric acid for 60 s showed the most degradation of collagen, which was 4. 86 （1.55） mg/g, followed by 30 s group and 15 s group, which were 2.76（0.87） mg/g and 1.93（0.88） mg/g, respectively. Group of 10 s was 0.95（0. 38） mg/g. The control group showed the least degradation of 0. 06（0. 03） mg/g. Significant differences in collagen degradation were found among groupo （P 〈 0. 005）. Smear layer were removed well but tubular orifices and collagen fibrils were covered by particles after dentin

关 键 词：酸蚀 牙 牙本质 胶原Ⅰ型 羟脯氨酸 

分 类 号：R781[医药卫生—口腔医学]