心理应激对大鼠实验性牙周炎及牙周组织中低氧诱导因子1α表达的影响  

Effect of psychological stress on development of periodontitis and expression of periodontal hypoxia-inducible factor 1α in rats

作　　者：张增方[1,3] 吕芳丽[2] 陈莹[2] 杨晓燕[2] 黄绮凌[1] 李东健 黄世光[1] 

机构地区：[1]暨南大学医学院口腔医学系,广东广州510632 [2]中山大学中山医学院寄生虫学教研室,中山大学热带病防治研究教育部重点实验室,广东广州510080 [3]广州市妇女儿童医疗中心口腔科,广东广州510120 [4]广州市荔湾区口腔医院,广东广州510170

出　　处：《中国病理生理杂志》2011年第2期315-319,共5页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.81071387);广东省自然科学基金资助项目(No.10151008901000006)

摘　　要：目的:探讨心理应激对牙周炎及其氧代谢的影响。方法:建立大鼠实验性牙周炎模型并予以应激刺激,SPF级4周龄雄性Wistar大鼠48只,随机分为4组:(1)正常对照组;(2)实验性牙周炎组:用浸有牙周致病菌的丝线结扎左侧上颌第二磨牙牙颈部复制牙周炎模型;(3)单纯应激组:牙周不作特殊处理,给予应激刺激;(4)牙周炎+应激组:按上法复制牙周炎模型,并予以应激刺激。各组动物分别于实验后第1、4、6、8周末分批处死,各时点每组处死的动物数为3只。大鼠处死前测量牙周附着丧失,制作牙体牙周联合切片观察牙周的组织学改变;采用免疫组化法检测各实验组大鼠牙周组织低氧诱导因子1α(HIF-1α)的表达水平,通过计算HIF-1α平均阳性细胞率来评估组织的缺氧程度。结果:(1)与正常对照组比较,单纯应激组牙周附着丧失程度在各时点均无显著差异(P>0.05);(2)牙周炎+应激组牙周附着丧失程度在4、6和8周时明显高于牙周炎组(P<0.01);(3)与正常对照组比较,单纯应激组HIF-1α平均阳性细胞率在各时点均无显著差异(P>0.05);(4)牙周炎+应激组HIF-1α平均阳性细胞率在4、6和8周时显著高于牙周炎组(P<0.01)。结论:应激刺激可能通过降低牙周组织氧代谢水平加重大鼠牙周炎症程度。AIM： To investigate the effect of psychological stress on the development of periodontitis and the expression of periodontal hypoxia - inducible factor let（ HIF - 1α） in rats. METHODS： Forty - eight male Wistar rats （ SPF grade） were randomly divided into 4 groups： （ 1 ） normal control group, i.e. naive rats; （2） experimental periodonti- tis group ： the periodontitis model was induced by wrapping 3 - 0 silk ligature inoculated with putative periodontopathic bacteria around the left maxillary second molar of the rats; （3） stress group： the rats were treated with stress alone;（4） periodontits with stress group, the periodontitis model was induced as above, and the rats were treated with stress. The rats were sacrificed at week 1,4, 6 and 8 after the ligature. The attachment losses（AL） were measured by home - made probe. The histological changes of periodontal tissues stained with hematoxylin and eosin （HE） were observed under microscope. The HIF- 1α expression level in the periodontal epithelium was determined by immunohistochemistry that was used to evaluate the severity of hypoxia by measuring the average rate of HIF - 1 α- positive cells. RESULTS： No significant difference of AL between stress group and normal control group was observed （ P 〉 0. 05 ）. The AL and the average rate of HIF - 1 α - positive cells in periodontitis with stress group were significantly higher than those in experimental periodontitis group at time points of week 4,6 and 8 after ligature （both P 〈 0.01 ）. CONCLUSION： Psychological stress is one of the periodontitis inducing factors in the animal model. Psychological stress may aggravate periodontitis by decreasing tissue oxygenation in rats.

关 键 词：牙周炎 动物模型 心理应激 低氧诱导因子1Α 

分 类 号：R78[医药卫生—口腔医学]