复合不平衡氨基酸对荷肝癌H_(22)小鼠肿瘤的影响  

Effect of Complex Amino Acid Imbalance Solutions on Hepatocarcinoma in Tumor Bearing H_(22) Mice

作　　者：闫忠芳[1] 陆伟[2] 齐玉梅[3] 杨晶[3] 金玉坤[1] 钱绍诚[3] 

机构地区：[1]天津市海河医院营养科,天津市300350 [2]天津市传染病医院肝内科 [3]天津市第三中心医院营养科

出　　处：《中国肿瘤临床》2011年第3期134-137,共4页Chinese Journal of Clinical Oncology

基　　金：天津市卫生局科技基金资助(编号:05KY15)~~

摘　　要：目的:研究复合氨基酸失衡液及几种单成分氨基酸失衡液对荷瘤宿主肝癌抑制的影响。方法:以荷肝癌H_(22)ICR小鼠为研究对象,将小鼠随机分为9组:A组(平衡氨基酸)、B组(富含精氨酸)、C组(去缬氨酸)、D组(富含亮氨酸)、E组(去酪氨酸)、F组(去苯丙氨酸)、G组(去丝氨酸)、H组(不加氨基酸)、I组(复合氨基酸),分别给予不同组别的肠内营养液12天。以肿瘤体积、肿瘤质量、肿瘤质量/尸质量、抑瘤率、肿瘤细胞坏死率、PCNA指数以及DNA倍性作为观察肝癌抑制的指标。结果:B、C、D、E、F、G、H、I与A组比较,肿瘤体积、肿瘤质量、肿瘤质量/尸质量以及PCNA指数差异有统计学意义(P<0.05);各组肿瘤细胞坏死率、DNA倍性差异无统计学意义(P>0.05);细胞周期分析显示:H、I组与A组比较,G_0/G_1期细胞百分数均升高,S期和G_2/M期细胞百分数均下降(P<0.05)。结论:复合氨基酸失衡液及单成分氨基酸失衡液对肝癌生长均具有不同程度的抑制作用,而平衡氨基酸液则促进肝癌生长。Objectives： To study the influence of several amino acid imbalance solutions on hepatocarcinoma inhibition and nutri- tional status in tumor-bearing mice. Methods： Based on individual formulas of amino acid compositions in nutrient solutions, the hepatoma22-bearing mice were divided into 9 groups： group A （ amino acid balance solution ）, group B （a rginine-enriched amino acid imbalance solution）, group C （ valine-depleted amino acid imbalance solution ）, group D （ leucine-enriched amino acid imbalance solution ）, group E （tyrosine-depleted amino acid imbalance solution ）, group F （ phenylalanine-depleted amino acid imbalance solution ）, group G （ serine-depleted amino acid imbalance solution ）, group H （ no amino acid ）, and group I （ compound amino acid imbalance solution ）. Gastrointestinal nutrient solution was used for 12 days in the different groups of hepatoma22-bearing mice. Indices of hepatocarcinoma inhibition included tumor volume, tumor weight, tumor weight/carcass body weight, tumor cell necrosis rate, PCNA expression and DNA ploidy. Results： The factors used to assess tumor inhibition were significantly lower in groups B, C, D, E, F, G, H and I than in group A （ P 〈 0.05 ）, except for tumor cell necrosis and DNA ploidy （ P 〉 0.05 ）. Cell cycle analysis showed that in a compari- son of group H and I with group A, the percentage of cells in G0/G1 phase was increased in group H and I, while the percentage of cells in S phase and GYM phase was decreased in the two groups （ P 〈 0.01 ）. Conclusion： Balanced amino acid solution can stimulate tumor growth, while the complex amino acid imbalance solutions and some individual amino acid imbalance solutions have inhibitory effect on hepatocarcinoma in nude mice.

关 键 词：氨基酸失衡液 肠内营养 小鼠 肝癌 

分 类 号：R735.7[医药卫生—肿瘤]