当归多糖对放射损伤小鼠骨髓单个核细胞黏附分子表达及细胞周期的影响  

EFFECTS OF ANGELICA POLYSACCHARIDES ON EXPRESSION OF ADHESION MOLECULES AND CELL CYCLE IN BONE MARROW MONONUCLEAR CELLS OF RADIATION INJURED MICE

作　　者：张雁[1] 关雪晶[1] 吴宏[1] 周玥[1] 姜蓉[1] 何晓莉[1] 

机构地区：[1]重庆医科大学干细胞与组织工程研究室基础医学院组胚教研室,重庆400016

出　　处：《中国组织化学与细胞化学杂志》2010年第6期587-592,共6页Chinese Journal of Histochemistry and Cytochemistry

基　　金：重庆市自然科学基金计划项目(2007BB5284)

摘　　要：目的探讨当归多糖(APS)对放射损伤小鼠骨髓单个核细胞(BMNC)黏附分子表达及细胞周期的影响,旨在阐明APS防护辐射性造血损伤的分子机制。方法建立小鼠放射损伤模型后连续给予不同剂量APS 13d,在不同时间点进行外周血白细胞、红细胞、血小板及BMNC计数;流式细胞术检测小鼠Sca-1+BMNC黏附分子CD44和CD49d表达及BMNC细胞周期的变化;Western blot和RT-PCR方法分别检测小鼠BMNC细胞周期蛋白(Cyclin)D2 mRNA和蛋白表达水平的改变。结果与正常组比较,NS组外周血WBC、RBC、PLT及BMNC计数明显减少,Sca-1+BMNC CD44、CD49d表达明显下降,G0/G1期细胞比例显著增加,CyclinD2 mRNA和蛋白表达水平明显降低。2 mg/kgAPS组和8 mg/kgAPS组能增加外周血各指标及BMNC计数,第7d明显提高Sca-1+BMNC黏附分子CD44、CD49d的表达水平,第14d能降低Sca-1+BMNC黏附分子CD44、CD49d的表达水平,降低G0/G1期细胞比例,提高CyclinD2mRNA和蛋白表达水平。结论当归多糖能通过调节放射损伤小鼠Sca-1+BMNC黏附分子的表达水平、上调BMNC的CyclinD2 mRNA和蛋白表达水平来加速BMNC G1期向S期的转换,促进造血恢复。Objective To explore the effects of Angelica polysaccharides（APS） on the expression of adhesion molecules and the cell cycle in bone marrow mononuclear cells（BMNC） of radiation injured mice,so as to illuminate the molecular mechanism of APS sheltering radiation injured haematogenesis.Methods Different doses of APS were given for 13 days continuously after the establishment of the radiation-injured model.Peripheral blood cells and BMNC counts were made.Flow cytometry was used to detect the expression of CD44,CD49d of Sca-1＋BMNC and the cell cycle of BMNC.The mRNA and protein expressions of cyclin D2 in murine BMNC were assayed by RT-PCR and Western blot.Results Compared with those in the normal group,the numbers of the peripheral leukocytes,erythrocytes,platelets and BMNC in the NS group decreased significantly,the expression of CD44 and CD49d of Sca-1＋BMNC declined,the percentage of G0/G1phases significantly increased,and the mRNA and protein expressions of Cyclin D2 were obviously cut down.2 and 8mg/kg increased the numbers of peripheral blood cells and BMNC,obviously elevated the expression of CD44 and CD49d of Sca-1＋BMNC on the 7th day.and decreased on the 14th day,cut down the percentage of G0/G1 phases,and raised the mRNA and protein expressions of Cyclin D2.Conclusion APS can regulate the expression of CD44,CD49d on Sca-1＋BMNC,and promote the mRNA and protein expressions of CyclinD2 in radiation injured mice to accelerate the cell cycle from G1 phase to S phase,which can help the recovery of hematopoietic functions in mice.

关 键 词：当归多糖 SCA-1 黏附分子 CD44 CD49D 细胞周期 CYCLIN D2 小鼠 

分 类 号：R146[医药卫生—公共卫生与预防医学]