胶质瘤组织中Nanog基因的表达及其意义  

Expression and significance of Nanog in gliomas

作　　者：李冬雪[1] 牛朝诗[1,2,3] 刘于海[1] 高歌[1] 费小瑞[1] 李明武[1] 董永飞[1] 

机构地区：[1]安徽医科大学附属省立医院神经外科,安徽合肥230001 [2]安徽医科大学附属省立医院神经分子生物学与神经再生修复研究室,安徽合肥230001 [3]安徽医科大学附属省立医院安徽省脑立体定向神经外科研究所,安徽合肥230001

出　　处：《中华神经外科疾病研究杂志》2010年第6期504-508,共5页Chinese Journal of Neurosurgical Disease Research

基　　金：国家自然科学基金资助项目(30672166)

摘　　要：目的探讨Nanog基因在不同病理级别胶质瘤组织中的表达及其意义。方法采用免疫组织化学染色法和逆转录酶-聚合酶链反应(RT-PCR)方法从蛋白和mRNA水平检测50例不同病理级别胶质瘤组织和7例正常脑组织标本中Nanog的表达。结果 Nanog蛋白在胶质瘤组织WHOⅡ级、Ⅲ级、Ⅳ级的阳性细胞率分别为18.3%±9.6%、37.9%±19.0%、53.4%±19.8%,差异有统计学意义(F=14.918,P=0.000);NanogmRNA的相对含量在胶质瘤组织WHOⅡ级、Ⅲ级、Ⅳ级中分别为0.1824±0.0310、0.3730±0.0961、0.4594±0.0453,差异有统计学意义(F=65.901,P=0.000),Nanog蛋白和mRNA表达强度随着病理级别增高而升高,而在正常脑组织中均未见表达。结论 Nanog在胶质瘤组织中高表达,其表达强度与病理级别密切相关,为进一步研究其与肿瘤干细胞关系及其在胶质瘤的生物学行为中所发挥的作用奠定基础。Objective To explore the expression and significance of Nanog in gliomas of different pathological grades.Methods The expression of Nanog mRNA was examined by reverse transcription polymerase chain reaction （RT-PCR） and Nanog protein was detected by immunohistochemistry in the specimens of 50 cases of glioma tissues and 7 cases of normal brain tissues.Results The immunopositive ratio was 18.3%±9.6% in WHO Grade Ⅱ,37.9%±19.0% in WHO Grade Ⅲ,53.4%±19.8% in WHO Grade Ⅳ,respectively （F=14.918,P=0.000）.The relative mRNA level of Nanog was 0.1824±0.0310 in WHO Grade Ⅱ,0.3730±0.0961 in WHO Grade Ⅲ,0.4594±0.0453 in WHO Grade Ⅳ,respectively （F=65.901,P=0.000）.The relative expression level of Nanog obviously correlated with the pathological grades.The Nanog protein and mRNA were not detected in normal brain tissues.Conclusion Nanog is overexpressed in gliomas.The relative expression level of Nanog is related to the pathological grades,which lays a foundation to the further research on its relation with brain tumor stem cells and its role in biological behaviors of gliomas.

关 键 词：胶质瘤 NANOG 脑肿瘤干细胞 表达 

分 类 号：R739.41[医药卫生—肿瘤]