新基因nelin在下肢曲张静脉中的表达及其意义  

Expression and roles of nelin in varicose veins

作　　者：于晓强[1] 秦士勇[1] 陈士辉[1] 张曙光[1] 

机构地区：[1]山东大学附属千佛山医院血管外科,山东济南250014

出　　处：《中国现代普通外科进展》2010年第12期929-932,共4页Chinese Journal of Current Advances in General Surgery

基　　金：山东省自然科学基金(ZR2009CL018)

摘　　要：目的:从基因的转录和翻译水平探讨nelin在下肢曲张静脉中的表达及意义。方法:分别利用RT-PCR技术以及免疫组织化学技术比较静脉曲张患者(实验组)以及正常人(对照组)之间大隐静脉血管组织中nelin基因及其蛋白的表达水平。结果:实验组的病变静脉组织中,nelin的表达低于对照组。RT-PCR显示,实验组与对照组目的条带亮度不同,对照组较实验组亮;免疫组织化学显示,对照组中nelin蛋白大量分布于正常静脉管壁血管平滑肌细胞的胞质,呈棕黄色,平均阳性细胞百分比为(80.67±10.20)%;而实验组中染色较弱,呈浅黄色,平均阳性细胞百分比为(18.39±7.78)%,差异有统计学意义(P<0.01)。结论:与正常人相比,原发性下肢静脉曲张患者的病变静脉组织中nelin基因在转录和翻译水平均存在明显的表达下调,这可能与病变静脉组织的形态学改变有一定的关系。Objective： Previous studies found that nelin,as a new gene,specifically expressed in cardiac and vascular smooth muscle.In this study,we testified the alteration of nelin expressing style at gene transcription and translation levels,to explore the expression of nelin in varicose veins and presume its roles.Methods： Then reverse transcription（RT-PCR） and Immunohistochemical techniques were applied to investigate the expression level of nelin mRNA and protein in normal and abnormal veins.Results： In the abnormal veins isolated from the patients with varicose veins,the expression of nelin was significantly down-regulated,compared with that in control group.RT-PCR： the target band intensity of control group was more lighter than that of patients with varicose veins;Immunohistochemical study revealed that signals of nelin protein existed extensively and strongly in the cytoplasm of smooth muscle cells in the normal veins,but scarcely and weakly in the study group.Percentage of positive cells： 80.67%±10.20% vs 18.39%±7.78%,P0.01.Conclusion： The expression of nelin gene is greatly down-regulated at both transcription and translation levels in the abnormal veins isolated from the patients with varicose veins.This phenomenon may be associated with the vascular remodeling of involved veins.

关 键 词：静脉曲张 nelin基因 下肢 

分 类 号：R735.7[医药卫生—肿瘤]