艾芬地尔对大鼠脑缺血再灌注后Survivin表达的影响  

Effect of Ifenprodil on Survivin Expression After Cerebral Ischemia-Reperfusion

作　　者：刘学娟[1] 杨金升[2] 郑佳丽[1] 马亚杰[1] 

机构地区：[1]兰州大学第二临床医学院 [2]兰州军区兰州总医院神经内科,甘肃省兰州市730050

出　　处：《中国动脉硬化杂志》2010年第11期865-867,共3页Chinese Journal of Arteriosclerosis

摘　　要：目的探讨大脑中动脉缺血再灌注后缺血侧海马区Survivin的表达规律及艾芬地尔对大鼠脑缺血再灌注后不同时相Survivin表达的影响。方法 72只Wistar大鼠随机分为假手术组、模型组和艾芬地尔干预组,采用线拴法制作局灶性大鼠脑缺血再灌注模型,假手术组于术后,模型组和艾芬地尔干预组于脑缺血2 h分别再灌注3 h、24 h、72 h及7天后处死取材,免疫荧光法测量不同时相缺血侧海马神经元Survivin的表达。结果假手术组Survivin呈现少基础量表达,模型组和艾芬地尔干预组Survivin表达规律基本一致,即在再灌注3 h时表达即有升高,至72 h达到高峰,7天表达有所下降,与假手术组比较差异显著(P<0.05);艾芬地尔干预组荧光强度值明显高于对应模型组(P<0.05)。结论大脑中动脉缺血再灌注后Survivin的表达升高,72 h达高峰;艾芬地尔对大脑中动脉缺血再灌注损伤具有保护作用。Aim To investigate the expression discipline of Survivin after perifocal areas of cerebra after ischemia-reperfusion（I/R） in rats,and to identify the effect of ifenprodil on Survivin after I/R. Methods Seventy-two Wistar rats were randomly divided into sham group,model group and ifenprodil group.The right middle cerebral artery occlusion reperfusion（MCAO/R） model was established by thread ligation.After operation in sham group,2 hours ischemia of middle cerebral artery was followed with 3 hours,24 hours,72 hours and 7days reperfusion in model group and ifenprodil group.Rats were sacrificed and Survivin in hippocampus of ischemia side was detected by immunofluorescence. Results Fluorescence intensity of the sham group was very weak,showing the basal expression;while fluorescence intensity of model group and ifenprodil group had almost the same trend,which expression rose 3 hours after I/R,increased continuously and reached the peak at 72 hours,and then decreased afterward,and there were significant differences compared with the sham group（P0.05）.Fluorescence intensity of ifenprodil group were stronger than the model group at the same time point（P0.05）. Conclusion Expression of Survivin increased after MCAO/R,reaching the maximum at 72 hours.Ifenprodil had a protective effect on the damage of I/R.

关 键 词：缺血再灌注 SURVIVIN 艾芬地尔 

分 类 号：R363[医药卫生—病理学]