胃肠道间质瘤中Ezrin过表达的临床病理学意义  

Over expression of Ezrin and its clinicopathologic significance in gastrointestinal stromal tumors

作　　者：余丽 刘勇[2] 徐元成 戴艳枝[2] 

机构地区：[1]浙江省永康市第一人民医院病理科,321300 [2]江西省人民医院病理科,南昌330006

出　　处：《临床与实验病理学杂志》2010年第6期666-669,共4页Chinese Journal of Clinical and Experimental Pathology

摘　　要：目的应用组织芯片技术检测Ezrin在胃肠道间质瘤(gastrointe stinals tromal tumors,GIST)中的过表达情况,并分析其临床病理学意义。方法采用免疫组化及原位杂交法检测组织芯片中42例GIST组织Ezrin蛋白和mRNA的过表达情况,并进行数据统计分析。结果在42例GIST组织中,21例有Ezrin蛋白的过表达(50%),16例有Ezrin mRNA的过表达(38%);Ezrin蛋白和mRNA在发生于非胃部位的GIST及出现浸润转移的患者中过表达更明显(P<0.05);此外Ezrin蛋白的过表达在肿瘤大小分组及恶性潜能分级、临床分期中有差异,差异有统计学意义(P<0.05);Ezrin蛋白和mRNA表达呈正相关(r=0.392,P<0.05)。结论 Ezrin可能参与了GIST的浸润转移过程,并可能成为预测GIST转移的重要指标;免疫组化检测Ezrin蛋白的过表达可以作为评估GIST恶性潜能及肿瘤进展的指标。Purpose To investigate the Ezrin overexpression and its relationship with clinicopathologic parameters in gastrointestinal stromal tumors（GISTs） by using tissue microarray.Methods The overexpression of Ezrin protein and mRNA was detected by immunohistochemistry and in situ hybridization,respectively,in 42 cases of GISTs.The data were statistically analyzed.Results 21（50%）and 16 cases（38%）of GISTs showed Ezrin protein and mRNA overexpression.Ezrin was frequently overexpressed in GISTs,especially those arising from the non-gastric sites and those with invasion and metastasis.Ezrin protein overexpression had difference between the subgroups of tumor size and NIH risk category and clinical stage（P0.05）.There was a positive correlation between Ezrin protein and mRNA overexpression.Conclusions Ezrin may be involved in the process of invasion and metastasis in GISTs,and it may be an important predictor for the metastasis in GISTs.Ezrin protein overexpression might become an indicator for evaluating the malignant potential and progression of GISTs.

关 键 词：胃肠道肿瘤 间质瘤 组织芯片 EZRIN 免疫组织化学 原位杂交 

分 类 号：R735.2[医药卫生—肿瘤] R735.3[医药卫生—临床医学]