耐盐异养硝化菌驯化方法及分离菌株鉴定  

Domestication of Halotolerant Heterotrophic Nitrifying Bacteria and Identification of Two Isolated Strains

作　　者：张培玉[1] 曲洋[1] 杨瑞霞[1] 郭沙沙[1] 于德爽[1] 

机构地区：[1]青岛大学环境科学与工程系,青岛266071

出　　处：《应用与环境生物学报》2011年第1期121-125,共5页Chinese Journal of Applied and Environmental Biology

基　　金：国家自然科学基金项目(Nos.50678085;50878107);青岛大学优秀研究生学位论文培育项目(No.YSPY2009014)资助~~

摘　　要：采用高C/N、逐渐降低DO、菌体对数生长期前期转接以及增加培养液中海水比例的方法完成了耐盐异养硝化菌的富集和驯化,并从中分离筛选出两株高效异养硝化菌qy37和gs2.通过对两株菌的形态观察、生理生化试验以及16S rRNA序列分析,确定菌株qy37和菌株gs2分别为假单胞菌属(Pseudomonas)和盐单胞菌属(Halomonas).A new method for enrichment and domestication of halotolerant heterotrophic nitrifying bacteria was studied.The enrichment and domestication were accomplished by adopting high C/N ratio,gradually reducing DO,inoculating domestic bacteria to new culture medium in log phase and increasing the proportion of sea water for culture medium.Furthermore,two trains of heterotrophic nitrifying bacteria,qy37 and gs2 were isolated from enrichment and domestication system.Based on their morphological and physiological characters,as well as their sequence analyses of the 16S rRNA gene,strain qy37 and strain gs2 were identified as Pseudomonas sp.and Halomonas sp.respectively.

关 键 词：富集 驯化 异养硝化菌 同步脱氮 耐盐 污水处理 

分 类 号：X172[环境科学与工程—环境科学]