乌司他丁对脑缺血再灌注损伤大鼠血脑屏障通透性和MMP-9活性的影响  

Effect of ulinastatin on the permeability of the blood-brain barrier and the activity of MMP-9 on rats with focal cerebral ischemia/reperfusion injury

作　　者：李青[1] 白宏英[1] 曾志磊[1] 王波[1] 

机构地区：[1]郑州大学第二附属医院神经内科,河南郑州450014

出　　处：《中风与神经疾病杂志》2011年第2期123-125,共3页Journal of Apoplexy and Nervous Diseases

摘　　要：目的观察乌司他丁对大鼠局灶脑缺血再灌注损伤后血脑屏障(BBB)通透性和基质金属蛋白酶-9(MMP-9)活性的影响。方法采用栓线法制备大鼠局灶性脑缺血再灌注损伤模型,腹腔注射乌司他丁,于脑缺血再灌注后6h、24h、48h、72h处死大鼠,通过测定损伤侧脑组织中伊文思蓝(EB)含量来观察BBB通透性的改变,用明胶酶谱法检测同侧脑部MMP-9活性变化。结果脑缺血再灌注损伤后,大鼠EB含量增加,24h最明显;MMP-9活性明显升高,48h达高峰。乌司他丁处理组EB含量及MMP-9活性水平明显低于脑缺血再灌注组(P<0.05)。结论乌司他丁可抑制脑缺血再灌注后大鼠MMP-9的活性,减轻BBB通透性的破坏。Objective To investigate the effect of ulinastatin（UTI） on the permeability of the blood-brain barrier and the activity of matrix metalloproteinase-9（MMP-9） on rats with focal cerebral ischemia/reperfusion（I/R） injury.Method With a model of reversible middle cerebral artery occlusion（MCAO） in rats,UTI（10000U/kg） was injected intraperitoneally.Blood brain barrier（BBB） permeability evaluated by detection of extravasated Evans Blue（EB） and the MMP-9 activity measured by gelatin zymography were separately investigated over a time course ranging from 6h to 72h（n=6） of reperfusion following two hours of cerebral ischemia.Results The contents of EB increased progressively over time after I/R,and reached maximum levels by 24h（P0.05）.The activity of MMP-9 significantly elevated by 24h（P0.05） and reached the peak after 48h following I/R.The contents of EB and the activity of MMP-9 in the UTI group were reduced significantly in comparison with the ischemia/reperfusion（IR） group（P0.05）.Conclusion UTI may suppress the activity of MMP-9 and mitigate the permeability of the blood-brain barrier on rats with focal cerebral ischemia/reperfusion injury.

关 键 词：乌司他丁 基质金属蛋白酶9 血脑屏障 明胶酶谱 

分 类 号：R743.3[医药卫生—神经病学与精神病学]