A型钾通道Kv1.4在致痫大鼠海马区的表达  

Expresion of A-type potassium channel Kv1.4 in the hippocampi regions of epilepsy model

作　　者：李亚伟[1] 曾常茜[2] 邹飒枫[1] 胡波[1] 李冬平[1] 

机构地区：[1]大连大学附属新华医院,辽宁大连116021 [2]大连大学医学院,辽宁大连116622

出　　处：《中风与神经疾病杂志》2011年第2期133-136,共4页Journal of Apoplexy and Nervous Diseases

基　　金：大连市科技基金项目(2008J21JH005)

摘　　要：目的通过检测A型钾通道Kv1.4在戊四唑(PTZ)致痫大鼠海马CA1、CA3及齿状回区的表达变化,探讨A型钾通道与癫痫发病的关系。方法 SD大鼠40只,随机分为对照组、致痫后1h、24h、72h组,每组各10只。腹腔注射PTZ制备大鼠癫痫模型,应用免疫组化及Western Blot技术检测Kv1.4在各时间段海马CA1、CA3及齿状回区的蛋白表达。結果致痫组大鼠海马区Kv1.4蛋白水平在致痫后1h、24h、72h 3个时间段均明显低于正常组(P<0.05);各致痫组之间Kv1.4蛋白水平均无明显差异(P>0.05)。结论 (1)A型钾通道Kv1.4在SD大鼠海马中广泛分布,表达丰富,以轴突处最为明显。(2)大鼠癫痫模型海马区A型钾通道Kv1.4蛋白表达减少,提示Kv1.4的表达下调可能与癫痫的发病相关。Objective This article aimed to check the expression of A-type potassium channel Kv1.4 in the hippocampi CA1,CA3 and DG regions of epilepsy model-induced by pentylenetetrazol（PTZ）,and discuss the relation of A-type potassium channel to epilepsy.Methods Forty SD rats were divided randomly into control group and epilepsy groups（1h,24h and 72h after injecting PTZ）,each group had ten rats.The rats models of epilepsy were established by injecting PTZ.Kv.1 4 protein were deteced at different times in the CA1,CA3 and DG regions of hippocampus,by using immunohistochemical assay and Western Blot.Results PTZ-induced epilepsy caused a obvious decline in Kv1.4 protein at 1h,24h and 72h time point（P0.05）;the expressions of Kv1.4 protein among the experimental groups were not obviously different（P0.05）.Conclusions A-type potassium channel Kv1.4 had a wide distributing in the hippocampus of SD rats,expressed abundantly and especially in neuraxon.The expression of A-type potassium channel Kv1.4 protein in hippocampus of epileptic model was descendant,and this result clewed that the downregulation may be associated with epilepsy.

关 键 词：A型钾通道 戊四唑 Kv1.4 

分 类 号：R742.1[医药卫生—神经病学与精神病学]