利用cDNA微阵列研究胃肠间质瘤耐药、复发相关基因的表达  

Analysis of recurrence-associated and imatinib resistance-associated gene expression in gastrointestinal stromal tumor by cDNA microarray

作　　者：闫竞一[1] 陈笑雷[1] 黄颖鹏[1] 韩少良[1] 周坤[1] 章圣辉[2] 

机构地区：[1]温州医学院附属第一医院胃肠外科,325000 [2]温州医学院附属第一医院外科研究所

出　　处：《中华实验外科杂志》2011年第3期373-376,共4页Chinese Journal of Experimental Surgery

基　　金：基金项目：温州市科技计划资助项目（Y20090289）

摘　　要：目的 观察复发与未复发胃肠间质瘤（GIST）间的基因表达差异,探讨恶性GIST伊马替尼耐药、复发发生发展机制.方法 收集5对患者的新鲜复发及未复发GIST组织随机配对成组行cDNA微阵列研究.应用荧光定量聚合酶链反应（PCR）方法对芯片结果进行验证.结果 复发与未复发GIST间的差异表达基因共1088个,其中表达上调者592个,下调者496个;上调基因功能主要与信号传导、肿瘤发生、药物代谢、细胞黏附等相关;下调基因功能则主要与细胞间隙连接通讯、肿瘤抑制、补体免疫防御等相关;GIST相关基因c-kit（CD117）、CD34在两组间差异均无统计学意义;荧光定量PCR验证结果与芯片表达结果一致.结论 运用cDNA芯片进行基因表达谱分析,有助于探讨GIST复发、伊马替尼耐药机制并寻找新的治疗靶标.Objective To study the differential expression of recurrence-associated and imatinib resistance-associated gene between recurrent and unrecurrent gastrointestinal stromal tumors （GIST） and explore the molecular mechanism of drug resistance and recurrence of malignant GIST by cDNA microarray analysis.Methods A total of 5 pairs of recurrent and unrecurrent fresh GIST tissues were subjected to cDNA microarray with 41000 genes.Array results were validated by reverse transcription-polymerase chain reaction （RT-PCR）.Results There were 1088 differential expression genes between recurrent and unrecurrent GIST tissues,including 592 genes up-regulated and 496 down-regulated.Most of the overexpressed genes were related to signaling pathway,oncogenesis,drug metabolism,cell adhesion,and the down-regulated genes were related to gap junction,tumor suppression,complement imumune defense.GIST related genes （c-kit,CD34） had no significant difference between recurrent and unrecurrent GIST tissues.RT-PCR confirmed the aAbstract：Objective To study the differential expression of recurrence-associated and imatinib resistance-associated gene between recurrent and unrecurrent gastrointestinal stromal tumors （GIST） and explore the molecular mechanism of drug resistance and recurrence of malignant GIST by cDNA microarray analysis.Methods A total of 5 pairs of recurrent and unrecurrent fresh GIST tissues were subjected to cDNA microarray with 41000 genes.Array results were validated by reverse transcription-polymerase chain reaction （RT-PCR）.Results There were 1088 differential expression genes between recurrent and unrecurrent GIST tissues,including 592 genes up-regulated and 496 down-regulated.Most of the overexpressed genes were related to signaling pathway,oncogenesis,drug metabolism,cell adhesion,and the down-regulated genes were related to gap junction,tumor suppression,complement imumune defense.GIST related genes （c-kit,CD34） had no significant difference between recurrent and unrecurrent GIST tissues.RT-PC

关 键 词：胃肠间质瘤 CDNA微阵列 复发 伊马替尼 

分 类 号：R735[医药卫生—肿瘤]